Thermo Scientific™ Pierce™ 3x DYKDDDDK Peptide

Description

• Pierce 3X DYKDDDDK Peptide is a lyophilized peptide with the sequence Met- Asp-Tyr-Lys-Asp-His-Asp -Gly- Asp-Tyr-Lys-Asp-His-Asp -Ile-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys (M DYKDHD G DYKDHD I DYKDDDDK )
• It has a molecular weight of 2861.9 g/mol
• For use, reconstitute to 1.5 mg/mL in PBS
• This tricosa-peptide can be used to competitively elute DYKDDDDK-tagged fusion proteins from immobilized anti-DYKDDDDK affinity supports, especially when a DYKDDDDK-tagged protein is sensitive to low pH
• At a 1.5 mg/mL concentration, complete release of tagged protein can be achieved in 5–10 minutes at ambient temperature
• Note: Pierce 3X DYKDDDDK Peptide may interfere with protein determination assays and absorbance at 280 nm
• However, the peptide can be removed from the eluate by desalting (e.g., with Zeba Spin Desalting Columns, Cat
• No
• 89891).