Gibco™ ExpiSf™ CD Medium

Description

• Delivers high reproducibility and lot-to-lot reliability Facilitates high-level expression of baculovirus-infected cultures, achieving protein yields three times higher than traditional insect expression platforms Supports growth of suspension Sf9 cultures to densities of approximately 2 x 10 7 cells/mL, with >90% cell viability Supports high-density infection of suspension Sf9 cells with recombinant baculovirus Supports growth, transfection, and baculovirus infection of Sf9 cells in culture formats from 24-deep well plates to >3L in shaker flasks Is a ready-to-use formulation, with no supplementation required manufactured using an ISO 13485-certified process according to 21 CFR 820 medical device current good manufacturing practices (cGMP) ExpiSf™ CD Medium is a core component of the ExpiSf Expression System
• For highest level protein expression, the medium is used in combination with a matched protein expression enhancer, ExpiSf Enhancer, which is supplied as part of the ExpiSf Protein Production Kit (Cat
• No
• A3767806)
• Sf21 and High Five™ cells adapted to other commercially-available serum-free media may be adapted for growth in ExpiSf CD Medium; although, sequential adaptation is required and high-density growth isn’t guaranteed
• ExpiSf™ CD Medium is not recommended for adherent cell cultures
• Cells are typically grown in Erlenmeyer-style flasks on a shaker platform in a non-humidified, non-CO2 cell culture incubator.