Gibco™ CTS™ AAV-MAX Lysis Buffer

Description

• Gibco CTS AAV-MAX Lysis Buffer is a ready-to-use cell lysis reagent for the extraction of adeno-associated virus (AAV) particles from producer HEK293 cells
• The cell lysis is detergent-based, eliminating the need for mechanical cell disruption, providing a milder and easier alternative when isolating AAV particles from cell cultures
• The buffer is supplied as a 10X solution that can be directly added to HEK293 AAV production cultures to induce cell lysis
• Note: the formulation of this product differs from the formulation of our research-use-only AAV-MAX Lysis Buffer (Cat
• No
• A50520)
• This formulation reduces sample turbidity and precipitation formation post-clarification, including when samples are stored between clarification and subsequent downstream workflow steps
• Features of CTS AAV-MAX Lysis Buffer include: • Polysorbate 20-based formulation that does not contain Triton X-100 • Formulated with 20 mM MgCl 2 for convenience • Manufactured in conformity with cGMP for medical devices (21 CFR Part 820) and follows USP and European Pharmacopoeia (Ph
• Eur.) 5.2.12 recommendations
• Drug Master File Letter of Authorization and Regulatory Support File available upon request
• The CTS AAV-MAX Helper-Free AAV Production System is a scalable, high-titer platform for suspension AAV production with research-grade and CTS versions to enable seamless transition from research to commercial manufacturing
• Other components of the CTS AAV-MAX system include CTS Viral Production Cells 2.0 (Cat
• No
• A48400), CTS AAV-MAX Transfection Kit (A5427701), CTS Viral-Plex Complexation Buffer (A5145401), and Viral Production Medium, AGT (A5147101).