Thermo Scientific™ Pierce™ High Capacity Streptavidin Agarose

Description

• Pierce Streptavidin Agarose and UltraLink Resins supply immobilized streptavidin biotin-binding protein in different resin formats, binding capacities and package sizes to suit nearly any immunoprecipitation or affinity purification procedure involving biotinylated molecules
• The products use a recombinant form of streptavidin with a mass of 53 kDa and a near-neutral pI
• The resins are prepared by covalently coupling streptavidin to the porous agarose or UltraLink Biosupport using efficient and stable chemistries, resulting in Streptavidin Agarose and UltraLink Resins that are leach-resistant and stable at pH 2-11
• The products are excellent choices for a variety of small- or large-scale affinity purification applications involving biotinylated macromolecules, including separation of biotinylated molecules from samples and immunoprecipitation of antigens using biotin-labeled antibodies
• Highlights: Proven coupling chemistries – the methods used to prepare these resins are superior to typical cyanogen bromide (CNBr) immobilization, providing high functionality, low nonspecific binding and excellent leach-resistance Versatile resins – Agarose (polysaccharide-based) and UltraLink™ Biosupport (acrylamide-based) resins provide two different options for particular experimental conditions and are compatible with gravity-flow, spin and FPLC methods Available in multiple formats – choose from two resin types, regular or high capacity preparations, and small or large-scale package sizes (bulk sizes are also available) Superior performance – High Capacity Resin has 2 to 3 times higher binding capacity than alternative supplier products, allowing use of small amounts of resin for experiments Item-Specific Features: Agarose Resins are crosslinked 6% beaded agarose UltraLink Resins beaded acrylamide support (UltraLink Biosupport) Plus and High Capacity Resins have higher loading (i.e
• density) of immobilized streptavidin protein than the regular forms Recommended for: Purification of membrane antigens in conjunction with biotinylated monoclonal antibodies; Cell-surface labeling with biotinylation reagents, followed by precipitation with immobilized streptavidin; Purification of cell-surface glycoproteins using biotinylated Concanavalin A; Recovery of single-stranded DNA for dideoxy sequencing