Thermo Scientific™ HisPur™ Cobalt Superflow Agarose

Description

• Perform high-purity, large-scale purification of polyhistidine-tagged proteins with Thermo Scientific™ HisPur Cobalt Superflow Agarose, a tetradentate-chelating Superflow 6 support charged with divalent cobalt
• Additional Information and Online Ordering HisPur Cobalt Superflow Agarose is a highly crosslinked, durable resin that does not compress at flow rates used for medium- to large-scale FPLC purifications
• The resin holds up well to a variety of chemicals and pH values, and is compatible with common clean-in-place procedures
• Compared to nickel and other ligands commonly used for immobilized metal affinity chromatography (IMAC), cobalt provides greater specificity for His-tagged protein purification
• The specific binding of HisPur Cobalt Superflow Agarose results in purer elution fractions, reducing the need for extra polishing steps
• Highlights: Robust – highly crosslinked 6% agarose Superflow beaded support tolerates linear flow rates up to 1200cm/hour with minimal leaching of cobalt due to a tetradentate chelator High yields – binds 20mg of 6xHis-GFP per mL of resin at a linear flow rate of 150cm/hour, can bind greater than 30mg per mL of resin depending on flow rate High purity – specificity of cobalt binding to histidine-tag generally results in elution fractions with less than 10% contamination of non-specific proteins Compatible – maintains function after exposure to a wide variety of chemicals and pH values Cost effective – resin is stable through multiple cycles of cleaning and reuse Recommended for: Large scale FPLC purification of polyhistidine tagged proteins