Thermo Scientific™ Glutathione

Description

• Thermo Scientific™ Pierce Glutathione Agarose is a high-capacity, high-performance resin for affinity purification of GST-tagged fusion proteins from cellular lysates
• The high–quality support consists of glutathione that has been immobilized by its central sulfhydryl group via a 12–atom spacer arm to crosslinked 6% beaded agarose
• Purification of GST–fusion proteins using glutathione (GSH) agarose beads is well documented and adaptable to a variety of scales, column formats and specific applications
• Whether the purpose is to purify large amounts of recombinant protein from over–expressing E
• Coli lysates or to investigate protein interactions involving GST–tagged bait proteins, Pierce Glutathione Agarose is suitable for the task
• To accommodate these many uses, Pierce Glutathione Agarose is offered in several package sizes and formats
• These include three volumes of resin slurry, three sizes of centrifuge-ready columns, complete GST purification kits, and two sizes of FPLC–ready chromatography cartridges
• Highlights: High capacity – binds at least 25mg of recombinant GST protein per milliliter of resin High yield and purity – consistently purifies at least 10mg of GST – tagged protein per milliliter of resin with greater than 90% purity Cost-effective – resin is economically priced and can be reused at least five times without reduction in binding capacity and purification performance Versatile – works well to purify GST – fusion proteins from bacterial lysates or use with pre-purified GST – tagged proteins to pull down protein interactions Compatible – validated and effective for use with Thermo Scientific Pierce Cell Lysis Reagents to extract and purify from bacterial or mammalian cell cultures Flexible – available in multiple formats including bulk resin, spin columns, chromatography cartridges and convenient GST purification kits Available Formats: Resin Slurries: 10mL, 100mL, 500mL bottles Spin Columns: 0.2mL (microcentrifuge), 1mL, 3mL columns Purification Kits: complete kits in all three column sizes Chromatography Cartridges: 1mL and 5mL cartridges, compatible with automated liquid chromatography systems and with syringe processing Bulk and Custom: let us know your needs for bulk Glutathione Agarose Includes: Kits contain Pierce Glutathione Spin Columns, equilibration/wash buffer and glutathione to prepare elution buffer