Thermo Scientific™ Halt™ Protease Inhibitor Cocktail (100X)

Description

• Thermo Scientific™ Halt Protease Inhibitor Cocktails are ready-to-use concentrated stock solutions of protease inhibitors for addition to samples to prevent proteolytic degradation during cell lysis and protein extraction procedures
• Halt Protease Inhibitor Cocktails are 100X solutions containing optimized concentrations of six broad-spectrum protease inhibitors stabilized in high-quality dimethylsulfoxide (DMSO)
• The cocktails effectively inhibit serine-proteases, cysteine-proteases, aspartic acid-proteases and aminopeptidases that are typically present in cellular lysate samples
• The identity and concentration of each component is fully disclosed, leaving no uncertainties about variables and compatibility with experiments
• Regular and EDTA-free formulations provide for inhibition of metalloproteases and compatibility with 2D electrophoresis, respectively
• The stable, concentrated liquid cocktails are easy to use, and the several different package sizes make Halt Protease Inhibitor Cocktails exceptionally convenient for either small-scale or large-scale uses
• Highlights: Better than tablets – the refrigerator-stable, 100X liquid format is more effective and easier to use than individual inhibitors or other formats; just pipette exactly the amount you need No proprietary ingredients – fully disclosed formulation contains AEBSF, aprotinin, bestatin, E-64, leupeptin and pepstatin A in DMSO Multiple package sizes – choose among several package sizes, including single-use microtubes that keep protease inhibitors fresh and reduce the risk of reagent contamination Two popular formulations – regular formulation includes a separate vial of EDTA solution for optional metalloprotease inhibition; EDTA-free formulation ensures compatibility with isoelectric focusing Lysis buffer compatible – use with Thermo Scientific Pierce™ Cell Lysis Buffers or nearly any other commercial or homemade detergent-based lysis reagent Includes: Protease inhibitor cocktails with EDTA include separate 0.5M EDTA solution.