Thermo Scientific™ HisPur™ Ni-NTA Resin

Description

• Perform routine affinity purification of His-tagged fusion proteins with Thermo Scientific™ HisPur Ni-NTA Resin, a high-capacity, high-performance nickel-IMAC resin
• The specially prepared nickel-NTA support consists of beaded agarose derivatized with the nitrilotriacetic acid (NTA) chelation moiety and loaded with divalent nickel ions (Ni2+)
• The immobilized metal affinity chromatography (IMAC) resin provides exceptional binding capacity and performance for recombinant His-tagged protein purification
• HisPur Ni-NTA Resin is available in several package sizes of resin slurry, three sizes of centrifuge-ready columns, complete purification kits, two sizes of FPLC-ready chromatography cartridges, and 96-well filter plates for high through-put needs
• With these options, high-yield His-tag protein purification is possible at nearly any scale
• Highlights: High capacity – bind up to 60mg of 6xHis-tagged protein per milliliter of resin Versatile – purify proteins using native or denaturing conditions Compatible – use with Thermo Scientific Cell Lysis Reagents and a variety of buffer additives Flexible – available in multiple formats including bulk resin, spin columns and chromatography cartridges Cost-effective – reuse the same batch of resin at least five times Easy to use – pre-formulated buffers available for kit formats Available Formats: Resin Slurries – crosslinked 6% beaded agarose; 10mL, 100mL, 500mL bottles Spin Columns – 0.2mL (microcentrifuge), 1mL, 3mL columns Purification Kits – complete kits in all three column sizes Chromatography Cartridges – compatible with automated liquid chromatography systems and with syringe processing Bulk and Custom – let us know your needs for bulk HisPur Ni-NTA Resin Includes: Kits contain HisPur Ni-NTA Columns (store at 4°C)