PI88838
Thermo Scientific™ Pierce™ HA-Tag Magnetic IP/Co-IP Kit
Manufacturer: Thermo Scientific™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PI88838-Each-of-1 | In Stock | ₹ 67,977.42 |
PI88838 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 67,977.42
GST (18%): ₹ 12,235.936
Total Price: ₹ 80,213.356
Content And Storage
Sufficient For: 40 IP reactions using 25 μL of anti-HA magnetic beads• HA-tagged Positive Control (Part No. 26180X), 500 μL• Pierce Anti-HA Magnetic Beads, 1 mL• Pierce IP Lysis/Wash Buffer, 2 × 50 mL• Non-Reducing Sample Buffer (5X), 5 mL• Elution Buffer (pH 2.0), 5 mL• Neutralization Buffer (pH 8.5), 1 mLHA-tagged Positive Control ships separately—store at -70°C. Store remaining components at 4°C.
For Use With (Equipment)
KingFisher™ Flex, Magnetic Stand
Quantity
40 reactions
Target
Target-Specific
Sufficient For
40 Reactions
Assay
Affinity Purification
Product Line
Pierce™
For Use With (Application)
Pull-Down (Tag-Based), Immunoprecipitation (IP)
Type
Tag IP/Co-IP Kit
Format
Kit
Description
- Perform immunoprecipitation (IP) assays of HA-tagged proteins or co-IP reactions with HA-tagged bait proteins with the magnetic beads, positive control and reagents provided in the Thermo Scientific™ Pierce Magnetic IP/Co-IP Kit
- Additional Information and Online Ordering The complete Magnetic IP/Co-IP Kit can be used to perform 40 IP or Co-IP assays
- It includes magnetic beads coated with immobilized anti-HA antibody, lysis/wash buffer, low pH elution buffer, neutralization buffer, HA-tag positive control lysate, and non-reducing sample buffer
- Protocols are provided for both manual and automated workflows
- Highlights: Specific – highly specific anti-HA monoclonal antibody enables high yields of immunoprecipitation products Fast – Optimized protocol is completed in approximately 1 hour Low non-specific binding – stable, pre-blocked beads provide highly purified HA-tagged proteins Versatile – beads are compatible with manual and automated workflows (e.g., Thermo Scientific™ KingFisher Instruments) Convenient and easy – complete kit includes all necessary reagents and easy-to-follow instructions Includes: The kit contains magnetic beads immobilized with anti-HA antibody, lysis/wash buffer, low pH elution buffer, neutralization buffer, HA-tag positive control lysate, and non-reducing sample buffer
- Recommended for: High-throughput immunoprecipitation of recombinant HA-tagged proteins and co-IP of interacting proteins
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Content And Storage: Sufficient For: 40 IP reactions using 25 μL of anti-HA magnetic beads• HA-tagged Positive Control (Part No. 26180X), 500 μL• Pierce Anti-HA Magnetic Beads, 1 mL• Pierce IP Lysis/Wash Buffer, 2 × 50 mL• Non-Reducing Sample Buffer (5X), 5 mL• Elution Buffer (pH 2.0), 5 mL• Neutralization Buffer (pH 8.5), 1 mLHA-tagged Positive Control ships separately—store at -70°C. Store remaining components at 4°C.
For Use With (Equipment): KingFisher™ Flex, Magnetic Stand
Quantity: 40 reactions
Target: Target-Specific
Sufficient For: 40 Reactions
Assay: Affinity Purification
Product Line: Pierce™
For Use With (Application): Pull-Down (Tag-Based), Immunoprecipitation (IP)
Type: Tag IP/Co-IP Kit
Format: Kit
Content And Storage:
Sufficient For: 40 IP reactions using 25 μL of anti-HA magnetic beads• HA-tagged Positive Control (Part No. 26180X), 500 μL• Pierce Anti-HA Magnetic Beads, 1 mL• Pierce IP Lysis/Wash Buffer, 2 × 50 mL• Non-Reducing Sample Buffer (5X), 5 mL• Elution Buffer (pH 2.0), 5 mL• Neutralization Buffer (pH 8.5), 1 mLHA-tagged Positive Control ships separately—store at -70°C. Store remaining components at 4°C.
For Use With (Equipment):
KingFisher™ Flex, Magnetic Stand
Quantity:
40 reactions
Target:
Target-Specific
Sufficient For:
40 Reactions
Assay:
Affinity Purification
Product Line:
Pierce™
For Use With (Application):
Pull-Down (Tag-Based), Immunoprecipitation (IP)
Type:
Tag IP/Co-IP Kit
Format:
Kit
Content And Storage: __
For Use With (Equipment): __
Quantity: 1 mL
Target: __
Sufficient For: Binding at least 100μg GST-c-Myc (26kDa fusion protein)/mL of bead suspension
Assay: __
Product Line: __
For Use With (Application): __
Type: Magnetic Affinity Bead
Format: __
Content And Storage:
__
For Use With (Equipment):
__
Quantity:
1 mL
Target:
__
Sufficient For:
Binding at least 100μg GST-c-Myc (26kDa fusion protein)/mL of bead suspension
Assay:
__
Product Line:
__
For Use With (Application):
__
Type:
Magnetic Affinity Bead
Format:
__
Content And Storage: Store at 4°C.
For Use With (Equipment): __
Quantity: 5 mL
Target: c-Myc
Sufficient For: __
Assay: __
Product Line: Pierce™
For Use With (Application): __
Type: Magnetic Affinity Bead
Format: __
Content And Storage:
Store at 4°C.
For Use With (Equipment):
__
Quantity:
5 mL
Target:
c-Myc
Sufficient For:
__
Assay:
__
Product Line:
Pierce™
For Use With (Application):
__
Type:
Magnetic Affinity Bead
Format:
__
Content And Storage: Sufficient For: 40 IP reactions using 25 μL of anti-c-Myc magnetic beads• c-Myc-tagged Positive Control (Part No. 23633), 500 μL• Pierce Anti-c-Myc Magnetic Beads, 1 mL• Mag c-Myc IP/Co-IP Buffer-1, 50 mL• Mag c-Myc IP/Co-IP Buffer-2 (20X), 20 mL• Non-Reducing Sample Buffer (5X), 5 mL• Elution Buffer (pH 2.0), 5 mLStore at 4°C.
For Use With (Equipment): KingFisher™ Flex Magnetic Particle Processor, Magnetic Stand
Quantity: 1 Kit
Target: Target-Specific
Sufficient For: 40 Reactions
Assay: Affinity Purification
Product Line: Pierce™
For Use With (Application): Pull-Down (Tag-Based), Immunoprecipitation (IP)
Type: Tag IP/Co-IP Kit
Format: Kit
Content And Storage:
Sufficient For: 40 IP reactions using 25 μL of anti-c-Myc magnetic beads• c-Myc-tagged Positive Control (Part No. 23633), 500 μL• Pierce Anti-c-Myc Magnetic Beads, 1 mL• Mag c-Myc IP/Co-IP Buffer-1, 50 mL• Mag c-Myc IP/Co-IP Buffer-2 (20X), 20 mL• Non-Reducing Sample Buffer (5X), 5 mL• Elution Buffer (pH 2.0), 5 mLStore at 4°C.
For Use With (Equipment):
KingFisher™ Flex Magnetic Particle Processor, Magnetic Stand
Quantity:
1 Kit
Target:
Target-Specific
Sufficient For:
40 Reactions
Assay:
Affinity Purification
Product Line:
Pierce™
For Use With (Application):
Pull-Down (Tag-Based), Immunoprecipitation (IP)
Type:
Tag IP/Co-IP Kit
Format:
Kit