Thermo Scientific™ Pierce™ Phosphoprotein Enrichment Kit

Description

• Perform efficient enrichment of phosphorylated proteins derived from mammalian cells and tissues with Thermo Scientific™ Pierce Phosphoprotein Enrichment Kit
• The kit is an effective system for isolating the phosphoprotein fraction of mammalian cell and tissue lysates
• The included lysis-binding buffer and proprietary affinity resin produce superior yields with negligible non-specific binding
• The kit uses a convenient spin column format, and the resulting phosphorylated protein sample is compatible with downstream applications, including Western blotting, mass spectrometry (MS), 2D-PAGE, and protein arrays
• The affinity resin is also available as pre-packed chromatography cartridges for use with automated liquid chromatography (LC) systems
• Highlights: Specific – low nonspecific protein contamination from complex biological samples, such as cell culture lysate and mouse tissue extract Fast – easy spin format enables enrichment of phosphorylated proteins in less than 2 hours Superior yield – achieves higher yields than other commercially available phosphoprotein isolation kits Convenient format – complete kit offering pre-dispensed spin columns, buffers, reagents and ultrafiltration columns for enrichment Compatible – downstream applications include mass spectrometry, Western blotting and 2D-PAGE