Thermo Scientific™ NeuCode™ Lysine-080

Description

• Thermo Scientific NeuCode amino acids augment the level of multiplexing achievable for the metabolic labeling of proteins for mass spectrometry analysis
• NeuCode Lysine-080 (3,3,4,4,5,5,6,6-D8 L-Lysine-2HCl) may also be used with traditional SILAC to improve flexibility of multiplexing options or to reduce complexity of analysis
• Stable isotope labeling with amino acids in cell culture (SILAC) is a powerful method to identify and quantify relative differential changes in complex protein samples
• NeuCode metabolic labeling is similar to SILAC, but differs in that the labeling only utilizes heavy amino acids
• The increased multiplexing capability of NeuCode amino acids is possible through the use of mass defects from extra neutrons in the stable isotopes
• These small mass differences may be resolved on high resolution mass spectrometers (Thermo Scientific™ Orbitrap™ Elite™, Orbitrap™ Fusion™ Tribrid™, and Orbitrap™ Fusion™ Lumos™ Tribrid™ mass spectrometers)
• Use of only heavy amino acids eliminates the need for 100% incorporation of amino acids used for SILAC (both heavy and light), and may be especially useful for studies with primary cells
• NeuCode amino acids are used together with specialized cell culture media that are deficient in essential amino acids
• Heavy L-lysine is used for SILAC analysis of peptides that have been digested with trypsin or LysC
• NeuCode Lysine-080 may be used with 13C6 15N2 L-lysine (NeuCode Lysine-602) for duplex experiments and may be combined with light lysine and 4,4,5,5-D4 L-lysine for three-plex experiments
• Labeling efficiency—100% label incorporation into proteins of living cells without toxicity Compatible—may be multiplexed with existing SILAC amino acids Time-saving—not necessary to label to 100% incorporation if only using heavy amino acids High-quality supplements—heavy amino acids with >98% isotope purity