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Affinity Chromatography Column

Affinity Matrix

Agarose

Agarose/Salmon Sperm DNA

Antibody Column Pack

Chromatography Cartridge

Column

IgG Purification Kit

Magnetic Bead

PANSORBIN Cells

Pre-packed Columns

Protein A HRP

Protein A Sepharose

Protein A/G HP SpinTrap Buffer Kit

Protein G Spin Plate

Purification Buffer

Resin

Spin Column

Spin Kit

Spin Plate

>1 mg Human IgG well

>50 g IgG/L

≈50 mg Human IgG/mL

12 to 15 mg of Human lgG/mg solid

125 mg Human IgG/Column

20 mg/mL Human IgG Medium

20 mg/mL IgG Medium

25 mg Human IgG/Column

60 mg hIgG/mL @ 5 min residence (80 mg/mL @ 10 min residence)

FIO (Target >65 mg/mL)

-20°C

0°C to 5°C

100 mL of resin provided as a 200 mL 50% slurry. Upon receipt store at 4°C.

2°C to 30°C, 20% Ethanol

2°C to 8°C

2°C to 8°C, 20% Ethanol

25 mL of resin provided as a 50 mL 50% slurry. Upon receipt store at 4°C.

4°C

4°C to 30°C

4°C to 30°C, 20% Ethanol

4°C to 8°C, 20% Ethanol

4°C. Do not freeze.

5 mL of resin provided as a 10 mL 50% slurry. Upon receipt store at 4°C.

Ambient temperatures

Store in refrigerator (2–8°C).

Sufficient For: Purifying 6 to 8 mg mouse IgG per column• Protein A Columns, 1 mL, 5 columns• Protein A IgG Binding Buffer, 1 L• IgG Elution Buffer, 500 mL• Excellulose Desalting Columns, 5 mL, 5 columnsStore at 4°C.

Upon receipt store at 4°C

Upon receipt store at 4°C to 8°C. Do not freeze.

Upon receipt store at 4°C. Product shipped with an ice pack.

Upon receipt store columns at 4°C

Upon receipt store kit at 4°C

Upon receipt store kit at 4°C. These products are shipped at ambient temperature.

Upon receipt store product at 2°C to 8°C. Product is shipped at ambient temperature.

Upon receipt store product at 4°C to 8°C. Product is shipped at ambient temperature.

Upon receipt store product at 4°C. Product is shipped at ambient temperature.

Upon receipt store product at 4°C. Product is shipped with wet ice pack.

Upon receipt store product at 4°C. Products are shipped at ambient temperature.

Upon receipt store products at 4°C. Products are shipped at ambient temperature.

Upon receipt store resin at 4°C. Product is shipped at ambient temperature.

1 Cartridge

1 column

1 Column

1 g

1 Kit

1 L

1 mg

1 mL

1 Pack

1 Plate

1 x 1 mL

1 x 1 mL Box

1 x 5 mL

1 x 5 mL Box

1.0 mL

1.5 mL

10 Column Kit

10 columns

10 Columns

10 g

10 mL

100 mL

15 mL

16 Columns

2 Cartridges

2 Column Kit

2 Columns

2 mg

2 mL

2 Pk.

2 x 1 mL

2.5 mL

20 mg

20 mL

25 mL

250 mL

3 mL

4

5 Column Kit

5 columns

5 Columns

5 g

5 L

5 mg

5 mL

5 Pk.

5 x 1 mL

5 x 1 mL Box

5 x 5 mL

5 x 5 mL Box

50 g

50 mL

Complete packsize

Each

Affinity purification of HIS-tagged proteins. Can be adapted to chromatography systems such as AKTA.

Antibody Purification

Buffers to be used when performing antibody purification using immobilized Protein A.

Centrifugation

Centrifugation, Vacuum

Chromatin immunoprecipitation (ChIPs) assays

Chromatography System, Manual

Designed for use with a syringe, peristaltic pump, or chromatography system

Dot Blotting, ELISA, Western Blotting, Immunohistochemistry

ELISA

ELISA, IP, Purification

ELISA, IP, WB, Purification

ELISA, WB

For detection of immunglobulins, purification

For detection of immunoglobulins via affinity to the Fc region - typically IgG from many mammalian species

Immunoassays

Immunoprecipitation, Immunoaffinity Purification, Western Blotting

Monoclonal antibody purification

Purification of Antibodies

Purification of human, mouse and rabbit immunoglobins