7184492

Thermo Scientific™ L-Arginine-HCl, 13C6 for SILAC

Study protein expression by mass spectrometry with mass variants of lysine, arginine and other amino acids to perform stable isotope labeling with amino acids in cell culture.

Manufacturer: Fischer Scientific

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Description

L-Arginine-HCl, 13C6

Product Type

Amino Acids

Label or Dye

Mass Isotope of Amino Acid

Starting Material

Cells

Detection Method

Mass Spectrometry

Form

Solid

For Use With (Equipment)

Mass Spectrometer

Content And Storage

Store at room temperature.

Shipping Condition

Room Temperature

Final Product Type

Protein

Workflow Step

Protein Labeling

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Description

  • Thermo Scientific™ Heavy and Light Amino Acids are used to specifically analyze protein expression by mass spectrometry using stable isotope labeling with amino acids in cell culture (SILAC) quantification kits
  • SILAC is a powerful method to identify and quantify relative differential changes in complex protein samples
  • This approach entails the in vivo metabolic incorporation of “heavy“ 13 C- or 15 N-labeled amino acids into proteins followed by mass spectrometry (MS) for the accelerated and comprehensive identification, characterization and quantitation of proteins
  • Highlights: Efficient – 100% label incorporation into proteins of living cells Flexible – different isotopes of heavy and/or light amino acids for arginine, lysine, leucine and proline enable the quantitation of peptides derived from MS-grade proteases Multiplex capabilities – several alternative isotopes of arginine and lysine are available that allow the analysis of multiple treatment conditions in each experiment High-quality supplements – heavy amino acids with >99% isotope purity

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Study protein expression by mass sp...


Description:
L-Arginine-HCl, 13C6

Product Type:
Amino Acids

Label or Dye:
Mass Isotope of Amino Acid

Starting Material:
Cells

Detection Method:
Mass Spectrometry

Form:
Solid

For Use With (Equipment):
Mass Spectrometer

Content And Storage:
Store at room temperature.

Shipping Condition:
Room Temperature

Final Product Type:
Protein

Workflow Step:
Protein Labeling

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Description:
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Product Type:
Amino Acids

Label or Dye:
Mass Isotope of Amino Acid

Starting Material:
Cells

Detection Method:
Mass Spectrometry

Form:
Solid

For Use With (Equipment):
Mass Spectrometer

Content And Storage:
Store at room temperature.

Shipping Condition:
Room Temperature

Final Product Type:
Protein

Workflow Step:
Protein Labeling

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Description:
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Product Type:
Amino Acids

Label or Dye:
Mass Isotope of Amino Acid

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Cells

Detection Method:
Mass Spectrometry

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For Use With (Equipment):
Mass Spectrometer

Content And Storage:
Store at room temperature.

Shipping Condition:
Room Temperature

Final Product Type:
Protein

Workflow Step:
Protein Labeling