Invitrogen™ eBioscience™ Streptavidin Super Bright 780 Conjugate

Description

• Super Bright dyes are polymer-based fluorophores that are excited by the violet laser (405 nm) and emit fluorescence at the wavelength indicated in their name
• They are optimized for use in flow cytometry and allow for use of additional channels with violet excitation
• Super Bright dyes are fully compatible with other commonly used fluorescent molecules, eBioscience buffers and fixatives, viability stains, and reagents
• Super Bright 780 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 780 nm
• We recommend using a 780/60 bandpass filter or equivalent
• Please make sure that your instrument is capable of detecting this fluorochrome
• Applications tested Streptavidin Super Bright 780 Conjugate has been tested by flow cytometric analysis to detect biotinylated primary antibodies
• The amount of this conjugate that will stain a cell sample in a final volume of 100 μL is less than or equal to 0.125 μg
• Cell number should be determined empirically but can range from 10 5 to 10 8 cells/test
• It is recommended that the streptavidin be carefully titrated for optimal performance in the assay of interest
• Samples can be stored in IC Fixation Buffer or 1-step Fix/Lyse Solution for up to three days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation
• Clone specific performance after fixation should be determined empirically
• When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer to minimize any non-specific polymer interactions
• Please refer to the datasheet for Super Bright Complete Staining Buffer for more information
• In some experiments, we have observed that compensation values for Super Bright 780-conjugated antibodies are higher in the violet 450/50 channel when using UltraComp eBeads microspheres as compared to single-color stained cells
• In such circumstances, we would recommend setting compensation with cells
• We have also observed this in some experiments using AbC Total Antibody Compensation beads
• Super Bright polymer dyes are sold under license from Becton, Dickinson and Company.