Molecular Probes™ Streptavidin, R-Phycoerythrin Conjugate (SAPE) - premium grade - 1 mg/mL

Description

• The R-phycoerythrin (R-PE) conjugate of streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (R-phycoerythrin)
• Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin)
• Streptavidin R-PE is very valuable in biotin-streptavidin detection protocols developed for flow cytometry, microplate assays, and microarrays
• This R-PE streptavidin conjugate is a high-purity product (purity confirmed by HPLC) and is supplied as 1 mL of a 1 mg/mL solution
• Important Features of Streptavidin RPE: Ex/Em maxima of ∼ (496, 546, 565/578) nm 5 to 10 times brighter than organic dyes Very high solubility in aqueous solutions Free of all unconjugated streptavidin; and mostly free of unconjugated R-PE Ideal for flow cytometry, microplate assays, and microarrays Properties of R-phycoerythrin R-PE has a quantum efficiency of 78%, making it an incredibly bright reagent, useful where high sensitivity is needed
• Because of poor photostability, however, it is not recommended for fluorescence microscopy
• Find Biotinylated Conjugates We offer an extensive line of biotinylated conjugates for use in biotin-streptavidin detection strategies
• Use the primary antibody search tool to find biotinylated primary antibodies Use the secondary antibody selector tool to find biotinylated secondary antibodies and biotinylated anti-dye and anti-hapten antibodies For Research Use Only
• Not intended for any animal or human therapeutic or diagnostic use
• Related Links: Learn more about Avidin-Biotin Detection Learn more about the practical applications for Streptavidin R-PE