Molecular Probes™ Streptavidin, Alexa Fluor™ 514 conjugate

Description

• Alexa Fluor™ 514 streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor™ dye)
• Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin)
• Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays
• Alexa Fluor™ dye streptavidin conjugates are supplied as 1 mg lyophilized product or in 0.5 mL volumes of a 2 mg/mL solution
• Important Features of Alexa Fluor™ 514 Streptavidin Conjugates : • Alexa Fluor™ 514 streptavidin conjugate has Ex/Em maxima of ∼ (518/540) • Bright, photostable fluorescence • High solubility in aqueous solutions • Available in multiple colors • Ideal for western blots, flow cytometry, imaging and microscopy, microplate assays and more Properties of Alexa Fluor™ Dyes Alexa Fluor™ dyes are organic fluorescent dyes developed for better performance in imaging and other labeling protocols and exhibit improved photostability and brightness and improved solubility in aqueous solutions
• Available in a broad range of colors , these dyes are a good choice for most imaging applications
• Blocking Endogenous Biotin Naturally occurring biotins can interfere with biotin-streptavidin detection schemes
• For experiments involving fixed and permeabilized cells, try our Endogenous Biotin-Blocking Kit to minimize this interference
• For Research Use Only
• Not intended for any animal or human therapeutic or diagnostic use
• Related Links: Learn more about Avidin-Biotin Detection Learn more about Alexa Fluor™ Dyes Find out about other Labeled Streptavidin Conjugates Read Avidin and Streptavidin Conjugates-Section 7.6 in the Molecular Probes Handbook