05-516

Anti-Ras Antibody, clone RAS10

clone RAS10, Upstate®, from mouse

Manufacturer: Sigma Aldrich

Synonym(S): GTP- and GDP-binding peptide B, GTPase Hras, Ha-Ras1 proto-oncoprotein, Ras family small GTP binding protein H-Ras, Transforming protein p2, c-has/bas p21 protein, c-ras-Ki-2 activated oncogene, p19 H-RasIDX protein, transformation gene: oncogene HAMSV,

Select a Size

Pack Size SKU Availability Price
100 μG 05-516-100-μG In Stock ₹ 47,680.00

05-516 - 100 μG

₹ 47,680.00

In Stock

Quantity

1

Base Price: ₹ 47,680.00

GST (18%): ₹ 8,582.40

Total Price: ₹ 56,262.40

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

RAS10, monoclonal

species reactivity

rat, human, mouse

manufacturer/tradename

Upstate®

technique(s)

ELISA: suitableflow cytometry: suitableimmunocytochemistry: suitableimmunohistochemistry: suitableimmunoprecipitation (IP): suitablewestern blot: suitable

isotype

IgG2aκ

NCBI accession no.

NM_005343

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Description

  • General description: The tumor oncoproteins HRas, KRas, and NRas are rated as Ras protoncogenes. Point mutations within Ras genes are frequently detected in human malignancies and in experimentally induced tumors in animals.
  • Specificity: Other species unknown.
  • Immunogen: Purified recombinant Ras. Clone RAS10.
  • Application: Detect Ras using this Anti-Ras Antibody, clone RAS10 validated for use in ELISA, FC, IC, IH, IP & WB.
  • Quality: Routinely evaluated by western blot on RIPA lysate from human A431 carcinoma cells, human HFF, Jurkat, Raji, mouse 3T3, CTLL, rat L-6 or PC-12 cell lysates.Western Blot Analysis: 0.05 - 0.5 μg/mL of this lot detected Ras in RIPA lysate from human A431 carcinoma cells. A previous lot detected Ras in human HFF, Jurkat, Raji, mouse 3T3, CTLL, rat L-6 and PC- 12 cell lysates.
  • Target description: 21 kDa
  • Linkage: Replaces: CBL751
  • Physical form: Format: Purified
  • Analysis Note: ControlPositive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
  • Other Notes: Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
  • Legal Information: UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

SAFETY INFORMATION

WGK

WGK 2

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mouse

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purified immunoglobulin

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primary antibodies

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RAS10, monoclonal

species reactivity:
rat, human, mouse

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Upstate®

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IgG2aκ

NCBI accession no.:
NM_005343

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mouse

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primary antibodies

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RAS10, monoclonal

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mouse, rat, human

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flow cytometry: suitableimmunocytochemistry: suitable

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IgG2aκ

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