Anti-Themis Antibody

Description

• General description: Thymocyte-expressed molecule involved in selection (Themis) is a highly conserved protein that has been shown to serve a key role in regulating T-cell development through TCR signaling, especially through the moderation of phosphorylation and calcium influx. It is also a critical component in the later stages of thymocyte development through the regulation of positive/negative T-cell selection, and is required for integration and maintenance of signals necessary to T-cell lineage commitment and maturation. Themis expression is observed in thymus, spleen, thymocytes between Pre-TCR and checkpoints positive in selectin, and at low levels in mature T-cells.
• Specificity: This antibody recognizes Themis at the C-terminus.
• Immunogen: KLH-conjugated linear peptide corresponding to the C-terminus of mouse Themis.
• Application: Detect Themis using this Anti-Themis Antibody validated for use in WB, FC.
• Quality: Evaluated by Western Blot in mouse thymocyte tissue lysate.Western Blot Analysis: 0.1 µg/mL of this antibody detected Themis in 10 µg of mouse thymocyte tissue lysate.
• Target description: ~72 kDa observed. UniProtKB/Swiss-Prot Q8BGW0 (THMS1_MOUSE) entry describes 5 isoforms produced by alternative splicing: Isoform 1 at 72.780 kDa, Isoform 2 at 69.544 kDa, Isoform 3 at 68.293 kDa, Isoform 4 at 59.082 kDa, and Isoform 5 at 30.040 kDa
• Analysis Note: ControlMouse thymocyte tissue lysate
• Other Notes: Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.