Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301, FITC conjugate

Description

• General description: Histone H2A is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2A is involved with the structure of the nucleosomes of the ′beads on a string′ structure.
• Specificity: Broad species cross-reactivity is expected based on conservation of sequence homology.
• Immunogen: Epitope: Ser139
• Application: Immunocytochemistry: 2-4 μg/mL of a previous lot detected phospho-histone H2A.X in etoposide-treated HeLa cells fixed with 95% ethanol/5% acetic acid (10 minutes), followed by 1% formaldehyde, 0.25% Triton™ X-100 in TBS (5 min.).
• Quality: Evaluated by Flow Cytometry to detect Log phase Jurkat cells treated with staurosporine. Flow Cytometry: Log phase Jurkat cells were treated with staurosporine (1 μg/mL) for the indicated time. Histone H2A.X (Ser 139) phosphorylation was detected as described in the manual for the H2A.X Phosphorylation Assay Kit (Flow Cytometry), Catalog # 17-344. Cells were analyzed on a Becton-Dickinson FACS-Calibur flow cytometer.
• Target description: 15 kDa
• Physical form: Protein G Purified
• Storage and Stability: Stable for 1 year at from date of receipt.
• Analysis Note: ControlStaurosporine-treated Jurkat cells
• Other Notes: Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
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• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.