Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor™ 488 conjugate

Description

• General description: Tubulins, major components of the cellular cytoskeleton, form microtubules that are critical for cellular structure, migration, cell division and intracellular trafficking. There are at least three types of tubulins, alpha, beta and gamma. Each has a molecular weight of approximately 55 kDa. Microtubules are large structures (300 angstrom diameter) composed of alternating alpha and beta subunits. Compounds that block microtuble formation, such as Taxol and the vinca alkaloids, inhibit cell division and are effective anti-cancer agents.
• Specificity: Recognizes α-Tubulin, clone DM1A, Mr 50-60 kDa.
• Immunogen: Epitope: The epitopehas been mapped to amino acids 426-450 (AALEKDYEEVGVDSV-EGEGEEEGEE).
• Application: Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor 488 conjugate is an antibody against α-Tubulin for use in FC, IC, IF & WB.
• Quality: Routinely evaluated by Flow Cytometry.Flow Cytometry: 0.2 μg of this lot detected α-Tubulin in fixed and permeabilized rat L6, Jurkat, and A431 cells.
• Target description: 50-60 kDa
• Physical form: Alexa Fluor™ 488 conjugated, purified mouse monoclonal IgG1 in buffer containing PBS containing 1% BSA, 0.05% Tween-20, 0.05% sodium azide. Liquid at 2-8°C.
• Storage and Stability: Stable for 1 year at 2-8°C from date of receipt. Do Not Freeze. Do not store the material diluted.For maximum recovery of product, centrifuge original vial prior to removing cap.
• Analysis Note: ControlNegative Control: Catalog # 16-240, Normal Mouse IgG, Alexa Fluor™ 488-conjugate.
• Other Notes: Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
• Legal Information: ALEXA FLUOR is a trademark of Life Technologies