Anti-Horse IgG (whole molecule)−Peroxidase antibody produced in rabbit

Description

• General description: Horse IgGs have seven subclasses ranging from IgG1 to IgG7. Equine IgG antibodies mainly regulate mucosal and systemic immunological responses and thereby, provide protection against disease-causing pathogens such as Streptococcus equi, and the horse flu virus. Horse IgG may also function to control the advancement of EHV-1 infection . Anti-horse IgG (whole molecule)−peroxidase antibody is specific for IgG in horses.
• Immunogen: Horse IgG
• Application: Anti-horse IgG (whole molecule)−peroxidase antibody may be used in dot blot (1:8,000-1:10,000), immunohistochemistry (formalin-fixed, paraffin-embedded sections, at 1:150 dilution) and direct ELISA (1:15,000).
• Biochem/physiol Actions: IgG, a monoclonal antibody can be cleaved at the hinge region by nonspecific proteases like papain and pepsin. This can result in univalent Fab (antigen-binding fragments) fragments or bivalent F(ab′)2 fragments.  These two enzymes have a broad substrate specificity resulting in heterogenous fragments.
• Physical form: Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT
• Preparation Note: Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.