Anti-RINF (CXXC5)

Description

• General description: CXXC-type zinc finger protein 5 (UniProt: Q7LFL8; also known as CF5, Putative MAPK-activating protein PM08, Putative NF-kappa-B-activating protein 102, Retinoid-inducible nuclear factor, RINF) is encoded by the CXXCS (also known as HSPC195, TCCCIA00297) gene (Gene ID: 51523) in human. In human cells, two isoforms of RINF have been reported that are produced by alternative splicing. The isoform two lacks the first 95 amino acids. RINF serves as a transcription factor that binds to the oxygen responsive element of COX4I2 and represses its transcription under hypoxia conditions (4% oxygen), as well as normoxia conditions (20% oxygen). Its nuclear localization sequence is localized in amino acids 257-262. It is shown to co-localize with DVL1 in large bodies localized outside the nuclear membrane. RINF is shown to be important in normal myelopoiesis. RINF acts as a mediator of BMP4-mediated modulation of canonical Wnt signaling activity in neural stem cells. It is also shown to be essential for DNA damage-induced ATM phosphorylation, p53 activation, and cell cycle arrest. RINF silencing is reported to increase sensitivity of K562 cells to daunorubicin- and lenalidomide. (Ref.: Aston A et al. (2013). Oncotarget. 4(9); 1438-1448).
• Specificity: This rabbit polyclonal antibody detects CXXC-type zinc finger protein 5 (RINF) in human cells. It targets an epitope within 17 amino acids from the N-terminal half.
• Immunogen: KLH-conjugated linear peptide corresponding to 17 amino acids from the N-terminal half of human CXXC-type zinc finger protein 5. Immunogen is conserved between two isoforms.
• Application: Anti-RINF (CXXC5) Antibody, Cat. No. ABE570, is a highly specific rabbit polyclonal antibody that targets RINF (CXXC5) and has been tested in Western Blotting.
• Quality: Evaluated by Western Blotting in MCF-7 cell lysate.Western Blotting Analysis: 0.5 µg/mL of this antibody detected RINF (CXXC5) in 10 µg of MCF-7 cell lysate.
• Target description: ~35 kDa observed; 32.98 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
• Other Notes: Concentration: Please refer to lot specific datasheet.