Anti-Methyl-Glutamine

Description

• General description: Glutamine methylation occurs on translation termination factors and ribosomal proteins. Protein methylation has been reported to affect various cellular processes including protein stability, protein-protein interaction, and protein localization. Methylation of histone is suggested as a platform to recruit and regulate other chromatin-related factors. Glutamine methylation is brought about by the action of protein glutamine methyltransferase HEMK2 that specifically requires a GQX3R motif for methylation activity. This antibody was tested for glutamine methylation in chromodomain helicase DNA-binding protein 5 (CHD5) that contains multiple PHD and chromo domains and is reported to function as a tumor suppressor.
• Specificity: This rabbit polyclonal antibody detects methylglutamine in proteins.
• Immunogen: KLH-conjugated Gly-Gln (Me)-Gly tripeptide.
• Application: Anti-Methyl-Glutamine, Cat. No. ABS2185, is a rabbit polyclonal antibody that detects glutamine methylated proteins. It has been tested for use in Western Blotting.
• Quality: Evaluated by Western Blotting in methylated recombinant chromodomain helicase DNA-binding 5 (CHD5) protein, but not in unmethylated recombinant CHD5.Western Blotting Analysis: A 1:3,000 dilution of this antibody detected Methyl-Glutamine in methylated recombinant chromodomain helicase DNA-binding 5 (CHD5) protein, but not in unmethylated recombinant CHD5.
• Target description: ~51 kDa observed (for methylated CHD5). Uncharacterized bands may be observed in some lysate(s).
• Physical form: Antiserum was depleted by running over unmethylated protein column.
• Other Notes: Concentration: Please refer to lot specific datasheet.