Anti-PICALM

Description

• General description: Phosphatidylinositol-binding clathrin assembly protein (UniProt: Q13492; also known as PICALM, Clathrin assembly lymphoid myeloid leukemia protein) is encoded by the PICALM (also known as CALM) gene (Gene ID: 8301) in human. PICALM is ubiquitously expressed protein that co-localizes with clathrin in the Golgi area. Five isoforms of PICALM have been reported that are produced by alternative splicing. PICALM acts as an assembly protein that recruits clathrin and adapter protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. It may also play a role in determining the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. It is reported to be involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction. PICALM variants are associated with Alzheimer′s disease and are thought to be involved in the endocytosis and recycling of the amyloid protein precursor and it can affect Alzheimer s disease disk primarily by modulating production, transportation, and clearance of beta-amyloid peptides. (Ref.: Xu, W., et al. (2015). Mol Neurobiol. 52(1);399-413).
• Specificity: This rabbit polyclonal antibody detects Phosphatidylinositol-binding clathrin assembly protein (PICALM) in human cells. it targets an epitope within 16 amino acids from the C-terminal half. It recognizes all 5 isoforms of PICALM.
• Immunogen: Epitope: C-terminus
• Application: Anti-PICALM Antibody, Cat. No. ABT288, is a rabbit polyclonal ntibody that detects PICALM in nhuman cells and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
• Quality: Evaluated by Western Blotting in HeLa cell lysate.Western Blotting Analysis: A 1:500 dilution of this antibody detected PICALM in HeLa cell lysate.
• Target description: ~61 kDa observed; 70.76, 68.76, 66.39, 59.92, and 70.04 kDa calculated, respectively for isoforms 1 to 5. Uncharacterized bands may be observed in some lysate(s).
• Physical form: Affinity Purified
• Storage and Stability: Stable for 1 year at 2-8°C from date of receipt.
• Other Notes: Concentration: Please refer to lot specific datasheet.
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.