Goat Anti-Rabbit IgG Antibody, (H+L) HRP conjugate

Description

• General description: Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein and mainly helps in immune defense. IgG is a major class of immunoglobulin. Rabbit IgG is further divided into five classes- IgM, IgG, IgA, IgD and IgE. It has only one IgG variant with one interheavy disulfide bridge.
• Specificity: Specific for rabbit IgG, heavy and light chain. The cross-reactivities of anti-rabbit IgG antibody are tested in an ELISA. Minimum cross-reactivity to human IgG.
• Application: Goat Anti-Rabbit IgG Antibody, (H+L) HRP conjugate has been used in:immunohistochemistry (IHC)western blottingimmunoprecipitation
• Biochem/physiol Actions: Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. It helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.
• Quality: Western Blot, chromogenic: 1:200-1:500 dilution can be used (Coligan, J., 1997; Harlow, E., 1988).Western Blot, chemiluminescent: 1:2,000-1:5,000 dilution can be used (Coligan, J., 1997).
• Physical form: ImmunoAffinity Purified
• Storage and Stability: The undiluted antibody is stable at 2-8°C for 12 months. Do not store in a diluted format.
• Legal Information: CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.