Goat Anti-Mouse IgM+IgG+IgA (H+L) Antibody, Alkaline Phosphatase, Species Adsorbed

Description

• General description: IgG is the most abundant immunoglobulin compose of two heavy chains and two light chains. Each molecule has two antigen binding sites. IgG has 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%). IgM constitutes about 10% of serum immunoglobulins. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. Monomeric IgA constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces.
• Specificity: Reacts with heavy and light chains of mouse IgM, IgG1, IgG2a, IgG2b, IgG3 and IgA. Absorbed for human sera and purified human paraproteins. Minimal cross reactivity with human immunoglobulins.
• Application: ELISA: 1:2,000-1:4,000 Optimal working dilutions must be determined by the end user.
• Physical form: Purified by affinity chromatography on pooled mouse IgM+IgG+IgA covalently linked to agarose. Liquid in 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative.
• Storage and Stability: Maintain refrigerated at 2°-8°C under sterile conditions for up to twelve months from date of receipt. Store long term at -20°C.
• Legal Information: CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany