Goat Anti-Mouse IgG Antibody, F(ab′)2, FITC conjugate

Description

• General description: Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
• Specificity: Specific for mouse IgG, heavy and light chain. The cross-reactivities of the anti-mouse IgG antibody were tested in an ELISA. Minimum cross-reactivity to human IgG.
• Application: Immunohistochemistry: 1:200-1:500 (Coligan et al. 1997; Harlow & Lane 1988; Bullock & Petrusz 1982; Javois 1994) Immunocytochemistry: 1:200-1:500 (Coligan et al. 1997; Harlow & Lane 1988; Bullock & Petrusz 1982; Javois 1994) Flow cytometery: 1 μg per 1 x 10E6 cells (Coligan et al. 1997; Harlow & Lane 1988; Javois 1994) FITC absorption peak is 488-492nm, its emission peak is 520nm. Optimal working dilutions must be determined by the end user.
• Physical form: ImmunoAffinity Purified
• Storage and Stability: The antibody conjugate solution is stable at 2–8°C for 12 months. Do not store in a diluted format.
• Legal Information: CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.