Anti-Mouse IgG (Fab specific) F(ab′)2 fragment–FITC antibody produced in goat

Description

• General description: IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections.
• Specificity: Binds all mouse Igs.Useful when trying to avoid background staining due to the presence of Fc receptors. Anti-Mouse IgG (Fab specific) F(ab′)2 fragment-FITC antibody recognizes Fab fragment of mouse IgG. No cross reaction is observed with the Fc fragment of mouse IgG. No reactivity is observed with human, horse or bovine serum. The antibody is purified by affinity isolation and conjugated with FITC in an alkaline reaction.
• Immunogen: Purified mouse IgG; The F(ab′)2 fragment of the antibody is obtained from pepsin digested antiserum
• Application: Anti-Mouse IgG (Fab specific) F(ab′)2 fragment−FITC antibody produced in goat has been used in:flow cytometry for fluorescence-activated cell sorting (FACS) analysisimmunofluorescence and confocal microscopyimmunofluorescent HSP72 detection/immunocytochemistry experiments
• Biochem/physiol Actions: Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reactions. IgG2a has cytotropic properties and IgG2 molecules have the ability to activate the complement system.
• Other Notes: Antibody adsorbed with bovine, equine and human serum proteins.
• Physical form: Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
• Preparation Note: Adsorbed to reduce background staining with bovine, horse or human samples.
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.