hGH ELISA

Description

• General description: Human growth hormone (hGH) is specifically detected by the hGH ELISA. It does not cross-react with rat growth hormone. Cross-reactivity with the human homologues TSH, FSH, and LH has not been tested. The hGH protein from E. coli, included in the kit for the purpose of compiling a standard calibration curve, is provided with lot-specific content data as determined by immunoassay.hGH StandardsThe hGH protein from E. coli, included in the kit for the purpose of compiling a standard calibration curve, is provided with lot-specific content data as determined by immunoassay.
• Specificity: The hGH ELISA specifically detecs human growth hormone (hGH). It does not cross-react with rat growth hormone.
• Application: For research use only. Not for use in diagnostic procedures.The hGH ELISA is used to quantitatively measure the expression level of hGH released into cell culture supernatant of eukaryotic cells transfected with a plasmid containing a reporter gene that encodes for hGH.[1][2]
• Features and Benefits: Standardized: Allows direct comparison of data from different sets of experiments, even when kits from different production lots are usedMore sensitive: The hGH ELISA is approximately 20-times more sensitive than isotopic hGH assaysMore specific: The hGH ELISA shows no crossreactivity with rat growth hormoneFaster: Measures hGH expression as early as 18hours post-transfection and requires only approximately 4 hours from start to finishEasy to perform: Follows a standard ELISA protocol
• Packaging: 1 kit containing 9 components.
• Specifications: Assay time: Approximately 4 hoursSample material: Culture supernatantSensitivity: ≥5pg/ml (≥1pg/well)Standards: The hGH protein from E. coli, included in the kit for the purpose of compiling a standard calibration curve, is provided with lot-specific content data as determined by immunoassay.
• Principle: The hGH ELISA is based on the sandwich ELISA principle. Antibodies to hGH (anti-hGH) are prebound to the surface of the microplate. The culture supernatant, which contains secreted hGH, is added to the wells of the microplate modules. All hGH contained in the medium binds to the anti-hGH bound to the microplate surface. Next, a digoxigenin-labeled antibody to hGH (anti-hGH-DIG) is added, then bound to hGH. In the following step, an antibody to digoxigenin conjugated to peroxidase (anti-DIG-POD) is added, then bound to digoxigenin. In the final step, the peroxidase substrate ABTS is added. The peroxidase enzyme catalyzes the cleavage of the substrate, yielding a colored reaction product. The absorbance of the sample is determined using an ELISA reader, and is directly correlated to the level of hGH present in the medium supernatant. The sensitivity of the assay can be enhanced using the peroxidase substrate (ABTS) with substrate enhancer.
• Preparation Note: Working solution: Solution 1 hGH Stock Solution (bottle 1)Reconstitute the lyophilizate in 0.5 ml double distilled water. The resulting concentration is calculated using the lot-specific information, given on the label (final conc. approx 10 ng/ml). This solution is used for the preparation of hGH standards for establishing a hGH calibration curve (see 7.2: preparation of hGH standards).Solution 2 Anti-hGH-DIG (bottle 2)Reconstitute the lyophilizate in 0.5 ml double distilled water (final conc. 100 μg/ml).Solution 2a Anti-hGH-DIG, working dilutionTo prepare anti-hGH-DIG working dilution, dilute the reconstituted anti-hGH-DIG solution (100 g/ml) with sample buffer (solution 7) to a final conc. of 1 μg/ml (e.g., 100 μl of reconstituted anti-hGH-DIG solution 9.9 ml of solution 7 for 50 wells).The working dilution should be prepared freshly before use and should not be stored.Solution 3 Anti-DIG-POD (bottle 3)Reconstitute the lyophilizate in 0.5 ml double distilledwater (final conc. 20 U/ml). Do not add sodiumazide!Solution 3a Anti-DIG-POD, working dilutionTo prepare anti-DIG-POD, working dilution, dilute the reconstituted anti-DIG-POD solution (20 U/ml) with sample buffer (solution 7) to a final conc. of 200 mU/ml (e.g., 100 μl of reconstituted anti-DIG-POD solution 9.9 ml of solution 7 for 50 wells). The working dilution should be prepared freshly before use and should not be stored.Solution 4 POD Substrate (bottle 4)Ready-to-use solution.Solution 5 POD Substrate containing Substrate Enhancer (bottles 4 and 5)If a low hGH concentration in the sample is expected, add 1 mg of substrate enhancer (bottle 5) per ml of solution 4 and mix by stirring for 30 min at 15 to 25 °C. The solution is stable for only 2 hours and should therefore be prepared immediately before use. Use the substrate enhancer only if the hGH concentration is low!Solution 6 Washing Buffer, 1× (bottle 6)To prepare a ready-to-use washing buffer, mix 1 part 10x washing buffer concentrate (bottle 6) with 9 parts of double distilled water.Solution 7 Sample Buffer (bottle 7)The solution is ready-to-use. Mix thoroughly before use. Do not add sodium azide.Storage conditions (working solution): Solution 1 hGH Stock Solution (bottle 1)The reconstitutedsolution is stable for 2 months at 2 to 8 °C. For long term storage, store in aliquots at or below -15 to -25 °C.Solution 2 Anti-hGH-DIG (bottle 2)The reconstituted solution is stable for two months at 2 to 8 °C. For long term storage, store in aliquots at or below -15 to -25 °C.Solution 2a Anti-hGH-DIG, working dilutionThe working dilution should be prepared freshly before use and should not be stored.Solution 3 Anti-DIG-POD (bottle 3)The reconstituted solution is stable for 6 months at 2 to 8 °C. Do not freeze! Solution 3a Anti-DIG-POD, working dilutionThe working dilution should be prepared freshly before use and should not be stored.Solution 4 POD Substrate (bottle 4)The solution is stable until the expiration date given on the label if stored at 2 to 8 °C.POD Substrate containing Substrate Enhancer (bottles 4 and 5)The solution is stable for only 2 hours and should therefore be prepared immediately before use.Solution 6 Washing Buffer, 1× (bottle 6)The reconstituted solution is stable for 6 months at 2 to 8 °C.Solution 7 Sample Buffer (bottle 7)After opening the bottle we recommend storing the solution in aliquots at -15 to -25 °C, since it does not contain a preservative agent.
• Analysis Note: No cross-reaction with rat growth hormone. Cross reactivity with the human homologues TSH, FSH, and LH has not been tested.
• Other Notes: For life science research only. Not for use in diagnostic procedures.