RAB0408

Mouse Prolactin ELISA Kit

for serum, plasma and cell culture supernatant

Manufacturer: Sigma Aldrich

Select a Size

Pack Size SKU Availability Price
1 KIT RAB0408-1-KIT In Stock ₹ 92,585.10

RAB0408 - 1 KIT

₹ 92,585.10

In Stock

Quantity

1

Base Price: ₹ 92,585.10

GST (18%): ₹ 16,665.318

Total Price: ₹ 1,09,250.418

species reactivity

mouse

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitablecapture ELISA: suitable

input

sample type plasmasample type serumsample type cell culture supernatant(s)

assay range

inter-assay cv: <12%intra-assay cv: <10%sensitivity: 30 pg/mLstandard curve range: 27.43-20000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

mouse ... Prl(19109)

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Description

  • General description: The Mouse Prolactin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Prolactin in serum, plasma, cell culture supernatants and urine.
  • Immunogen: Recombinant Mouse Prolactin
  • Application: For research use only. Not for use in diagnostic procedures.Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
  • Biochem/physiol Actions: Prolactin (Prl) is processed to lower molecular weight N-terminal fragments, 14-18kDa, called vasoinhibins, which have antiangiogenic effects. In endothelial cells, the 16kDa prolactin form exerts antiproliferative and proapoptotic effects which result in cell cycle arrest, caspase and NFκB (nuclear factor) activation and regulation of Bcl-2 protein family. In sexually mature female mice, the plasma levels of prolactin remain constant throughout estrous cycle, but show a sudden increase during proestrous cycle due to increase in circulating levels of estrogens.[1] Studies show that Prl activates T cells, B cells, natural killer (NK) cells, macrophages, neutrophils and dendritic cells and there is a significant level of hyperprolactinemia incidence in patients with autoimmune diseases, such as rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). Studies in rats show that Prl is involved in T cell homing to mammary glands during early lactation and that hypoprolactemia during lactation results in modified T cell distribution with increased T cell in circulation and reduced in mammary glands. [2]
  • Other Notes: A sample Certificate of Analysis is available for this product.Please type the word sample in the text box provided for lot number.

SAFETY INFORMATION

Pictograms

GHS05

Signal Word

Warning

Hazard Statements

H290

Precautionary Statements

P234 - P390

Hazard Classifications

Met. Corr. 1

WGK

WGK 3

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Sigma Aldrich

RAB0408

for serum, plasma and cell culture ...


species reactivity:
mouse

packaging:
kit of 96 wells (12 strips x 8 wells)

technique(s):
ELISA: suitablecapture ELISA: suitable

input:
sample type plasmasample type serumsample type cell culture supernatant(s)

assay range:
inter-assay cv: <12%intra-assay cv: <10%sensitivity: 30 pg/mLstandard curve range: 27.43-20000 pg/mL

detection method:
colorimetric

shipped in:
wet ice

storage temp.:
−20°C

Gene Information:
mouse ... Prl(19109)

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species reactivity:
rat

packaging:
kit of 96 wells (12 strips x 8 wells)

technique(s):
ELISA: suitablecapture ELISA: suitable

input:
sample type serumsample type cell culture supernatant(s)sample type plasma

assay range:
inter-assay cv: <12%intra-assay cv: <10%sensitivity: 400 pg/mLstandard curve range: 0.512-50 ng/mL

detection method:
colorimetric

shipped in:
wet ice

storage temp.:
−20°C

Gene Information:
rat ... Prlr(24684)

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Gene Information:
human ... PROK1(84432)

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assay range:
inter-assay cv: <12%intra-assay cv: <10%sensitivity: 200 pg/mLstandard curve range: 0.343-250 ng/mL

detection method:
colorimetric

shipped in:
wet ice

storage temp.:
−20°C

Gene Information:
human ... PSG2(5670)