ANTI-FLAG® M1 Agarose Affinity Gel

Description

• General description: ANTI-FLAG® M1 Agarose Affinity Gel is a purified mouse IgG2B monoclonal antibody covalently attached to agarose.
• Specificity: Binding specificity: Free N-Terminus of FLAG sequenceN-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C
• Application: For purification of N-terminal FLAG fusion proteins. Since binding is Ca2+-dependent, proteins can be eluted with a buffer containing EDTA, as well as by the standard methods using either FLAG peptide or glycine-HCl buffer, pH 3. ANTI-FLAG M1 does not bind to Met-FLAG fusion proteins, so this resin is not appropriate for purifying unprocessed, cytoplasmically expressed fusion proteins.Affinity gel is for calcium mediated purification of N-terminal FLAG fusion proteins.immunoprecipitation (IP): suitableElution - FLAG peptide, Glycine, pH 3.5 EDTALearn more product details in our FLAG® application portal.
• Features and Benefits: Typically purify fusion proteins from crude lysates to single band purity in just one chromatography step.Fusion protein may be eluted from affinity resin by mild elution with EDTA.A solution of FLAG peptide can be used for gentle, non-denaturing elution of FLAG fusion proteins.
• Physical form: Suspension of beaded agarose in 50% glycerol containing 10 mM sodium phosphate, 150 mM NaCl, pH 7.4, 0.02% (w/v) sodium azide
• Other Notes: ANTI-FLAG® M1 does not bind to Met-FLAG fusion proteins, so this resin is not appropriate for purifying unprocessed, cytoplasmically expressed fusion proteins.
• Legal Information: ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany