EZview™ Red Protein A Affinity Gel

Description

• General description: EZview™ Red Protein A Affinity Gel is a highly visible, red colored Protein A affinity gel. The affinity resin contains Staphylococcus aureus Protein A covalently attached to crosslinked 4% agarose beads. The affinity gel is used to bind the FC portion of the IgG of an antibody to samples, such as cell lysates and tissue. The immune complexes, bound to the EZview™ Red Protein A Affinity Gel, are recovered by centrifugation. Red dye enhances visibility for more efficient results.
• Application: EZview™ Red Protein A Affinity Gel has been used for purification of proteins in the preparation of immunoprecipitation[1][2] and chromatin immunoprecipitation (ChIP)[3] assays.
• Features and Benefits: Increased visibility - Red color reduces risk of incidental aspirationImproved recovery of target protein by reduced accidental lossHigher reproducibility - More consistent yields
• Physical form: 1:1 (v/v) suspension in PBS containing 50% glycerol and 15 ppm Kathon
• Storage and Stability: EZview™ Red Protein A Affinity Gel should be stored in 50% glycerol at -20 °C. This product is stable for at least one year when stored as indicated. Do not freeze in the absence of glycerol.
• Legal Information: EZview is a trademark of Sigma-Aldrich Co. LLC