Insect PopCulture® Reagent

Description

• General description: Insect POPCULTURE® Reagent is a detergent-based lysis reagent specifically formulated for total insect cell culture extraction without the need for centrifugation. The improved method recovers both protein released into the medium and intracellular protein, increasing processing efficiency and target protein yields (Loomis 2002). It is amenable for automated expression-level screening and is fully compatible with the Ni-NTA His•Bind<TMSYMBOL></TMSYMBOL> affinity purification method. Insect PopCulture reagent can be used for protein extraction from insect cells grown in suspension and adherent cells grown on tissue culture plates.
• Features and Benefits: No need to separate cells from culture mediaNo need to clarify cell extracts prior to purificationHigher protein yield due to target protein recovery from both medium and cellsDirect affinity adsorption of target proteins to IMAC affinity resin from the total culture extractCompatible with protease inhibitor cocktailsIdeal for high-throughput, expression-level screening and protein purificationCompatible with transfected and baculovirus-infected insect cell cultures
• Warning: Toxicity: Irritant (B)
• Other Notes: Loomis, K., et al. 2002. inNovations15, 16.
• Legal Information: This product is covered by EP Patent 1,432,822 owned by EMD Chemicals Inc. or its Affiliates.