NXTRACT
NuCLEAR™ Extraction Kit
For mammalian tissue or cultured cells
Manufacturer: Sigma Aldrich
Synonym(S): Nuclear Isolation Kit
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1 KIT | NXTRACT-1-KIT | In Stock | ₹ 47,575.88 |
NXTRACT - 1 KIT
In Stock
Quantity
1
Base Price: ₹ 47,575.88
GST (18%): ₹ 8,563.658
Total Price: ₹ 56,139.538
Quality Level
300
usage
kit sufficient for 10 extractions (1 ml packed cell volume) kit sufficient for 100 extractions (100 μl packed cell volume)
technique(s)
protein extraction: suitablewestern blot: suitable
shipped in
dry ice
storage temp.
−20°C
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Description
- General description: The procedure for the nuclear protein extraction method is to allow cells to swell with hypotonic buffer. The cells are then disrupted, the cytoplasmic fraction is removed, and the nuclear proteins are released from the nuclei by a high salt buffer.
- Application: NXTRACT kit was used to study the impact of salt on cardiac differential gene expression and coronary lesion in normotensive mineralocorticoid-treated mice.[1] It was also used to test the therapeutic potential of andrographolide for treating endometriosis. [2]
- Other Notes: Within this kit is a complete system for preparing nuclear and cytoplasmic protein extracts from mammalian tissue or cultured cells. All reagents necessary for extraction are included.
- Linkage: Recommended Antibodies for Immunodetection L1293, N2662, AMAB90549
- Legal Information: NuCLEAR is a trademark of Sigma-Aldrich Co. LLC
SAFETY INFORMATION
Pictograms
Signal Word
Danger
Hazard Statements
Precautionary Statements
P234 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363
Hazard Classifications
Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A
WGK
WGK 2
Flash Point(F)
188.6 °F
Flash Point(C)
87 °C
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Quality Level: 300
usage: kit sufficient for 10 extractions (1 ml packed cell volume) kit sufficient for 100 extractions (100 μl packed cell volume)
technique(s): protein extraction: suitablewestern blot: suitable
shipped in: dry ice
storage temp.: −20°C
Quality Level:
300
usage:
kit sufficient for 10 extractions (1 ml packed cell volume) kit sufficient for 100 extractions (100 μl packed cell volume)
technique(s):
protein extraction: suitablewestern blot: suitable
shipped in:
dry ice
storage temp.:
−20°C
Quality Level: __
usage: __
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shipped in: ambient
storage temp.: __
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usage:
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shipped in:
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shipped in: ambient
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shipped in:
ambient
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shipped in: __
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