DNase I

Description

• General description: DNase I is a double-strand specific endonuclease that degrades DNA. Bovine pancreatic deoxyribonuclease I (DNase I) is a DNA minor grove-interacting nuclease, which shows relatively low specificity. This protein is composed of two central β sheets, each composed of six β-strands. This structure is surrounded by extensive loop and α-helical regions. This enzyme shares structural similarity to exonuclease III.[1] DNase I is one of the most well characterized endonucleases of mammalian origin.[2]
• Application: DNase I has been used for tissue dissociation in conjunction with collagenases.[3][4][5]
• Biochem/physiol Actions: During tissue dissociation, parts of the cells are lysed resulting in a release of DNA. Monomolecular DNA may cause clumping of cells. Addition of DNase I to the dissociation buffer leads to a degradation of this extracellular DNA, therby avoiding the loss of cells from undesired clumping.[6][7][8]
• Features and Benefits: Lyophilized Filtered through 0.2 μm pore size membrane before lyophilization Glycoprotein and double-strand-specific endonuclease
• Unit Definition: One unit is the enzyme activity which yields an increase in absorbance of 0.001 per minute under the assay conditions.
• Preparation Note: Activator: Bivalent metal ions (Ca2+, Mg2+)Working concentration: 0.01 to 1 mg/mlNote: For each cell type the working concentration has to be determined individually. For optimal activity the enzyme needs 5 mM Mg2+.
• Reconstitution: Reconstitute the lyophylizate in sterile double-distilled water (10 mg/ml); further dilution with PBS (phosphate buffered saline), HBSS (Hank′s Balanced Salt Solution) or medium.
• Other Notes: For life science research only. Not for use in diagnostic procedures.