Description

• General description: Cellulase is a member of glycoside hydrolase family and is produced by a number of cellulolytic microbes.[1][2]
• Application: Cellulase from Aspergillus niger has been used:in the preparation of the standardized solution to determine absolute cellulase levels[3]in phosphate-citric acid buffer for activity assay[4]in non-starch polysaccharide degrading enzyme cocktail for in vitro hydrolysis of defatted rice bran and in the approach to improve the nutritive value of poultry feed[4]
• Biochem/physiol Actions: Cellulase from Aspergillus niger catalyzes the hydrolysis of endo-1,4-β-D-glycosidic linkages in cellulose, lichenin, barley glucan, and the cellooligosaccharides cellotriose to cellohexaose. It does not cleave cellobiose or p-nitrophenyl-β-D-glucoside. This enzyme will also cleave intact glycosaminoglycan from a core peptide by hydrolyzing the xylosyl serine linkage.
• Unit Definition: 1 U corresponds to the amount of enzymes which liberates 1 μmol glucose from carboxymethylcellulose per minute at pH 5.0 and 37°C
• Other Notes: View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer