Endo-β-Galactosidase, Bacteroides fragilis, Recombinant, E. coli

Description

• General description: Recombinant, Bacteroides fragilis endo-β-galactosidase expressed in E. coli. Endo-β-Galactosidase is an enzyme that hydrolyzes internal β-galactosidic linkages of oligosaccharides in poly-N-acetyl-lactosamine structures. This enzyme resembles the Escherichia freundii enzyme due to its specificity towards bovine corneal keratan sulphate, milk oligosaccharides and the glycolipids lacto-N-neotetraosylceramide and lacto-N-tetraosylceramide.
• Warning: Toxicity: Standard Handling (A)
• Unit Definition: One unit is defined as the amount of enzyme that will liberate one µmol reducing sugar from bovine corneal keratan sulfate per min at 37°C, pH 5.8.
• Physical form: Sterile-filtered in 20 mM Tris-HCl, pH 7.5.
• Reconstitution: There is no significant loss of activity if left at ambient temperature for several days. The enzyme is active for at least 5 days under the Recommended Reaction Conditions above.
• Other Notes: Murata, T., et al. 2003. Eur. J. Biochem.270, 3709.Scudder, P., et al. 1986. Eur. J. Biochem.157, 365.Scudder, P., et al. 1984. J. Biol. Chem.259, 6586.Scudder, P., et al. 1983. Biochem. J.213, 485.
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