63184

5-Maleimido-eosin

for fluorescence, ≥93% (HPLC)

Manufacturer: Sigma Aldrich

CAS Number: 150322-02-4

Synonym(S): EMA, Eosin-5-maleimide

Select a Size

Pack Size SKU Availability Price
10 MG 63184-10-MG In Stock ₹ 49,095.30
25 MG 63184-25-MG In Stock ₹ 86,402.40

63184 - 10 MG

₹ 49,095.30

In Stock

Quantity

1

Base Price: ₹ 49,095.30

GST (18%): ₹ 8,837.154

Total Price: ₹ 57,932.454

grade

for fluorescence

Quality Level

100

Assay

≥93% (HPLC)

fluorescence

λex 524 nm; λem 545 nm (after derivatization with 2-mercaptoethanol)

storage temp.

2-8°C

SMILES string

Oc1c(Br)cc2c(Oc3c(Br)c(O)c(Br)cc3C24OC(=O)c5cc(ccc45)N6C(=O)C=CC6=O)c1Br

InChI

1S/C24H9Br4NO7/c25-13-6-11-21(17(27)19(13)32)35-22-12(7-14(26)20(33)18(22)28)24(11)10-2-1-8(5-9(10)23(34)36-24)29-15(30)3-4-16(29)31/h1-7,32-33H

InChI key

YRCLWXRIEYQTAV-UHFFFAOYSA-N

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Description

  • General description: 5-Maleimido-eosin, also known as EMA or Eosin-5-maleimide, is a fluorescent binding agent. The 5-Maleimido-eosin binding test measures the EMA-labeled cells reduced mean channel fluorescence (MCF) reading.[1]
  • Application: Eosin-5-maleimide has been used as a fluorescent probe for the human erythrocyte band 3 protein. [2] It is widely used as a diagnostic probe for the laboratory detection of hereditary spherocytosis (HS) by flow cytometry. [3] The eosin-5-maleimide binding test was done to analyze the effect of alectinib (first line of treatment) for anaplastic lymphoma kinase (ALK).[4]
  • Other Notes: Triplet probe. Measurement of rotational diffusion of protein in solution and in membranes, including myosin and pyruvate dehydrogenase[5][6]

SAFETY INFORMATION

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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2-8°C

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Oc1c(Br)cc2c(Oc3c(Br)c(O)c(Br)cc3C24OC(=O)c5cc(ccc45)N6C(=O)C=CC6=O)c1Br

InChI:
1S/C24H9Br4NO7/c25-13-6-11-21(17(27)19(13)32)35-22-12(7-14(26)20(33)18(22)28)24(11)10-2-1-8(5-9(10)23(34)36-24)29-15(30)3-4-16(29)31/h1-7,32-33H

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YRCLWXRIEYQTAV-UHFFFAOYSA-N

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1S/C24H44P2/c1-21(2,3)25(22(4,5)6)17-19-15-13-14-16-20(19)18-26(23(7,8)9)24(10,11)12/h13-16H,17-18H2,1-12H3

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