Nitrotetrazolium Blue chloride
90.0-110.0% (calc. on dry substance, T), powder
Manufacturer: Sigma Aldrich
CAS Number: 298-83-9
Synonym(S): 2,2′-bis(4-Nitrophenyl)-5,5′-diphenyl-3,3′-(3,3′-dimethoxy-4,4′-diphenylene)ditetrazolium chloride, 3,3′-(3,3′-Dimethoxy-4,4′-biphenylene)bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride], p-Nitro-Blue tetrazolium chloride, p-Nitrotetrazolium blue, NBT, Nitro BT
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 100 MG | 93862-100-MG | In Stock | ₹ 5,629.00 |
| 500 MG | 93862-500-MG | In Stock | ₹ 19,539.13 |
| 2.5 G | 93862-2.5-G | In Stock | ₹ 77,701.85 |
93862 - 100 MG
In Stock
Quantity
1
Base Price: ₹ 5,629.00
GST (18%): ₹ 1,013.22
Total Price: ₹ 6,642.22
product name
Nitrotetrazolium Blue chloride, 90.0-110.0% (calc. on dry substance, T)
Quality Level
100
Assay
90.0-110.0% (calc. on dry substance, T)
form
powder
impurities
≤10% solvent content≤10% water
λ
in methanol
UV absorption
λ: 252-262 nm Amax
storage temp.
2-8°C
SMILES string
[Cl-].[Cl-].COc1cc(ccc1-[n+]2nc(nn2-c3ccc(cc3)[N+]([O-])=O)-c4ccccc4)-c5ccc(c(OC)c5)-[n+]6nc(nn6-c7ccc(cc7)[N+]([O-])=O)-c8ccccc8
InChI
1S/C40H30N10O6.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;;/h3-26H,1-2H3;2*1H/q+2;;/p-2
Other Options
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Description
- Biochem/physiol Actions: Nitrotetrazolium blue chloride (NBT) is used in staining, visualizing and evaluating the damage in brain sections or heart tissues. The principle of the technique is that the redox enzymes like nicotinamide adenine dinucleotide NAD(H) and cofactors present in the cell convert colorless tetrazolium salt to blue formazan. The dead cells remain unstained.[1] It is used in the dual-staining along with methyl green for the hyperspectral microscopic imaging analysis of colon tissues for the diagnosis of colon diseases.[2] NBT is also used for diagnosing microbial infections and phagocytic defects.[3] In conventional chromogenic methods, NBT/ 5-bromo-4-chloro-3-indolyl phosphate (BCIP) is used as a substrate for alkaline phosphatase enzyme reaction, in which NBT is reduced to insoluble purple diformazan.[4]
- Analysis Note: solubility:250 mg in 5 mL methanol: yellow to very dark yellow and light brown-yellow to very dark brown-yellow
SAFETY INFORMATION
Pictograms
Signal Word
Warning
Hazard Statements
Precautionary Statements
P261 - P264 - P270 - P301 + P312 - P302 + P352 - P305 + P351 + P338
Hazard Classifications
Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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product name: Nitrotetrazolium Blue chloride, 90.0-110.0% (calc. on dry substance, T)
Quality Level: 100
Assay: 90.0-110.0% (calc. on dry substance, T)
form: powder
impurities: ≤10% solvent content≤10% water
λ: in methanol
UV absorption: λ: 252-262 nm Amax
storage temp.: 2-8°C
SMILES string: [Cl-].[Cl-].COc1cc(ccc1-[n+]2nc(nn2-c3ccc(cc3)[N+]([O-])=O)-c4ccccc4)-c5ccc(c(OC)c5)-[n+]6nc(nn6-c7ccc(cc7)[N+]([O-])=O)-c8ccccc8
InChI: 1S/C40H30N10O6.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;;/h3-26H,1-2H3;2*1H/q+2;;/p-2
product name: __
Quality Level: 100
Assay: __
form: liquid (or paste)
impurities: __
λ: __
UV absorption: __
storage temp.: −20°C
SMILES string: __
InChI: __
product name: __
Quality Level: 300
Assay: __
form: __
impurities: __
λ: __
UV absorption: __
storage temp.: 2-8°C
SMILES string: Fc1ccc(cc1)[C@@]3(Cn2cncn2)O[C@@H]3c4ccccc4Cl
InChI: 1S/C17H13ClFN3O/c18-15-4-2-1-3-14(15)16-17(23-16,9-22-11-20-10-21-22)12-5-7-13(19)8-6-12/h1-8,10-11,16H,9H2/t16-,17+/m0/s1
