A3219
2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
peroxidase substrate, chromogenic, liquid
Manufacturer: Sigma Aldrich
Synonym(S): ABTS
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 100 ML | A3219-100-ML | In Stock | ₹ 14,137.45 |
A3219 - 100 ML
In Stock
Quantity
1
Base Price: ₹ 14,137.45
GST (18%): ₹ 2,544.741
Total Price: ₹ 16,682.191
product name
2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), Liquid Substrate System
Quality Level
200
form
liquid
storage temp.
2-8°C
SMILES string
CCN1C(\Sc2cc(ccc12)S(O)(=O)=O)=N\N=C3/Sc4cc(ccc4N3CC)S(O)(=O)=O
InChI
1S/C18H18N4O6S4/c1-3-21-13-7-5-11(31(23,24)25)9-15(13)29-17(21)19-20-18-22(4-2)14-8-6-12(32(26,27)28)10-16(14)30-18/h5-10H,3-4H2,1-2H3,(H,23,24,25)(H,26,27,28)/b19-17-,20-18-
InChI key
ZTOJFFHGPLIVKC-CLFAGFIQSA-N
Related Products
Description
- General description: The product is supplied as a ready-to-use one component substrate for peroxidase consisting 2,2′-azino-bis (3-ethylbenz-thiazoline-6-sulfonic acid) in an acidic buffer. The substrate is colorless before reacting with peroxidase and produces a blue-green color reaction product after reacting with it. 1% sodium dodecyl sulfate (SDS) may be used to stop the reaction during end-point assays. Since this substrate produces a soluble reaction product, it is not recommended for immunoblotting or histochemical applications.
- Application: 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) has been used as a peroxidase substrate in ELISA experiment. This substrate gives a green color soluble end product.[1][2] It has also been used as a colloidal preparation spread over the standard media plates, to evaluate the lignin oxidation activity. Presence of green coloration of the substrate exhibits microbial laccase activity.[3]
- Biochem/physiol Actions: 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) is a cation and undergoes oxidation in the presence of hydrogen peroxide. The oxidized ABTS is a blue-green product and is measurable colorimetrically.[4] A combination of ABTS, hydrogen peroxide and myoglobin is a standard system employed for measuring total antioxidant capacity(TAC) from samples.[5] ABTS method is flexible at varying pH and most widely used.[6] ABTS is a substrate for phenol oxidase enzyme.[7]
- Physical form: Ready-to-use.
SAFETY INFORMATION
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Compare Similar Items
Show Difference
product name: 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), Liquid Substrate System
Quality Level: 200
form: liquid
storage temp.: 2-8°C
SMILES string: CCN1C(\Sc2cc(ccc12)S(O)(=O)=O)=N\N=C3/Sc4cc(ccc4N3CC)S(O)(=O)=O
InChI: 1S/C18H18N4O6S4/c1-3-21-13-7-5-11(31(23,24)25)9-15(13)29-17(21)19-20-18-22(4-2)14-8-6-12(32(26,27)28)10-16(14)30-18/h5-10H,3-4H2,1-2H3,(H,23,24,25)(H,26,27,28)/b19-17-,20-18-
InChI key: ZTOJFFHGPLIVKC-CLFAGFIQSA-N
product name:
2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), Liquid Substrate System
Quality Level:
200
form:
liquid
storage temp.:
2-8°C
SMILES string:
CCN1C(\Sc2cc(ccc12)S(O)(=O)=O)=N\N=C3/Sc4cc(ccc4N3CC)S(O)(=O)=O
InChI:
1S/C18H18N4O6S4/c1-3-21-13-7-5-11(31(23,24)25)9-15(13)29-17(21)19-20-18-22(4-2)14-8-6-12(32(26,27)28)10-16(14)30-18/h5-10H,3-4H2,1-2H3,(H,23,24,25)(H,26,27,28)/b19-17-,20-18-
InChI key:
ZTOJFFHGPLIVKC-CLFAGFIQSA-N
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