C3142

α-Chymotrypsin from bovine pancreas

(TLCK treated to inactivate residual tryspin activity), Type VII, essentially salt-free, lyophilized powder, ≥40 units/mg protein

Manufacturer: Sigma Aldrich

CAS Number: 9004-07-3

Synonym(S): TLCK-Chymotrypsin

Select a Size

Pack Size SKU Availability Price
10 MG C3142-10-MG In Stock ₹ 8,125.20
25 MG C3142-25-MG In Stock ₹ 15,373.50
100 MG C3142-100-MG In Stock ₹ 40,670.40

C3142 - 10 MG

₹ 8,125.20

In Stock

Quantity

1

Base Price: ₹ 8,125.20

GST (18%): ₹ 1,462.536

Total Price: ₹ 9,587.736

Quality Level

200

type

Type VII

form

essentially salt-free, lyophilized powder

specific activity

≥40 units/mg protein

mol wt

25 kDa

solubility

1 mM HCl: soluble 10 mg/mL, clear

UniProt accession no.

P00767

storage temp.

−20°C

Gene Information

cow ... CTRB1(618826)

Other Options

Image Product Name Manufacturer Price Range
50-178-1454
Sigma Aldrich Fine Chemicals Biosciences alpha-Chymotrypsin from bovine pancreas suitable for protein sequencing, salt-free, lyophilized powder | 9004-07-3 | MFCD00130481 | 25UG
Sigma Aldrich Fine Chemicals Biosciences ₹ 14,254.30
50-178-1448
Sigma Aldrich Fine Chemicals Biosciences alpha-Chymotrypsin from bovine pancreas (TLCK treated to inactivate residual tryspin activity), Type VII, essentially salt-free, lyophilized powder, >=40 units/mg protein | 9004-07-3 | MFCD00130481 | 25MG
Sigma Aldrich Fine Chemicals Biosciences ₹ 13,311.42
50-178-1458
Sigma Aldrich Fine Chemicals Biosciences alpha-Chymotrypsin from bovine pancreas >=40 units/mg protein, vial of 5 mg | 9004-07-3 | MFCD00130481 | 10VL
Sigma Aldrich Fine Chemicals Biosciences ₹ 33,539.52
50-175-5654
Sigma Aldrich Fine Chemicals Biosciences alpha-Chymotrypsin from human pancreas lyophilized powder | 9004-07-3 | MFCD01323069 | 1VL
Sigma Aldrich Fine Chemicals Biosciences ₹ 71,682.17
50-178-1451
Sigma Aldrich Fine Chemicals Biosciences a-Chymotrypsin from bovine pancreas | 9004-07-3 | MFCD00130481 | 250mg
Sigma Aldrich Fine Chemicals Biosciences ₹ 8,699.74
50-186-9382
Sigma Aldrich Fine Chemicals Biosciences Chymotrypsin BRP, European Pharmacopoeia (EP) Reference Standard | 9004-07-3 |
Sigma Aldrich Fine Chemicals Biosciences ₹ 23,582.90
1134007
Chymotrypsin
Sigma Aldrich ₹ 48,784.50
C4129
α-Chymotrypsin from bovine pancreas
Sigma Aldrich ₹ 7,115.10 - ₹ 96,681.00
CHY5S
α-Chymotrypsin from bovine pancreas
Sigma Aldrich ₹ 34,154.70
C6423
α-Chymotrypsin from bovine pancreas
Sigma Aldrich ₹ 12,642.90 - ₹ 35,042.70

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Description

  • Application: α-Chymotrypsin from Sigma has been used to determine the crystal structures of two homologous inhibitors (pars intercerebralis major peptide-C and pars intercerebralis major peptide-D2v) from the insect Locusta migratoria by forming a complex with the enzyme.[1]
  • Biochem/physiol Actions: α-Chymotrypsin is a serine peptidase and has 241 amino acid residues contained in three polypeptide chains (A chain-13 residues, B chain-131 residues, and C chain-97 residues) linked by disulfide bridges. Molecular weight of this enzyme is found to be 25 kDa. Its pI is 8.75. It selectively hydrolyzes peptide bonds on the C-terminal side of tyrosine, phenylalanine, tryptophan, and leucine. Ca2+ activates and stabilizes the enzyme. The enzyme is inhibited by diisopropyl fluorophosphate (DFP), phenylmethanesulfonyl fluoride (PMSF), N-p-tosyl-L-phenylalanine chloromethyl ketone (TPCK), chymostatin, aprotinin, α1-antitrypsin, α2-macroglobulin, 10 mM Cu2+ and Hg2+.
  • Unit Definition: One unit will hydrolyze 1.0 μmole of BTEE per min at pH 7.8 at 25 °C.
  • Preparation Note: TLCK treatment inactivates trypsin which may be present in chymotrypsin, without affecting the chymotrypsin activity.
  • Analysis Note: Protein determined by A1%/280
  • Other Notes: View more information on chymotrypsin at www.sigma-aldrich.com/enzymeexplorer

SAFETY INFORMATION

Pictograms

GHS08,GHS07,GHS09

Signal Word

Danger

Hazard Statements

H302,H315,H319,H334,H335,H400

Precautionary Statements

P261 - P264 - P273 - P301 + P312 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

WGK

WGK 1

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Quality Level:
200

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Type VII

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essentially salt-free, lyophilized powder

specific activity:
≥40 units/mg protein

mol wt:
25 kDa

solubility:
1 mM HCl: soluble 10 mg/mL, clear

UniProt accession no.:
P00767

storage temp.:
−20°C

Gene Information:
cow ... CTRB1(618826)

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