C9658

Carboxypeptidase G from Pseudomonas sp.

lyophilized powder, ≥3 units/mg protein

Manufacturer: Sigma Aldrich

CAS Number: 9074-87-7

Synonym(S): γ-Glutamyl hydrolase

Select a Size

Pack Size SKU Availability Price
5 UNITS C9658-5-UNITS In Stock ₹ 25,274.70
20 UNITS C9658-20-UNITS In Stock ₹ 81,340.80

C9658 - 5 UNITS

₹ 25,274.70

In Stock

Quantity

1

Base Price: ₹ 25,274.70

GST (18%): ₹ 4,549.446

Total Price: ₹ 29,824.146

form

lyophilized powder

Quality Level

300

specific activity

≥3 units/mg protein

mol wt

homodimer ~90 kDa

composition

Protein, ~70% biuret

solubility

H2O: soluble 0.5 mg/mL

storage temp.

−20°C

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Description

  • Application: Carboxypeptidase G from Pseudomonas sp., or γ-Glutamyl hydrolase, has been used in a study to assess the role of the putidaredoxin COOH-terminus in P-450cam (cytochrome m) hydroxylations. Carboxypeptidase G from Pseudomonas sp. has also been used in a study to investigate the effects of nitric oxide on pemetrexed cytotoxicity via NO‑cGMP signaling in lung adenocarcinoma cells.
  • Biochem/physiol Actions: Carboxypeptidase G is a lysosomal, thiol-dependent protease, which progressively cleaves γ-glutamyl pteroyl poly-γ-glutamate yielding pteroyl-α-glutamate (folic acid) and free glutamate. It is considered highly specific for the γ-glutamyl bond, but not for the C-terminal amino acid of the leaving group.[1] Molecular mass of this homodimer is approximately 90 kDa. The enzyme is activated by Zn2+ ions.
  • Unit Definition: One unit will hydrolyze 1.0 μmole of L-glutamic acid from (+)amethopterin per min at pH 7.3 at 30 °C.
  • Physical form: Contains sodium acetate salt.
  • Preparation Note: Chromatographically purified

SAFETY INFORMATION

Pictograms

GHS08,GHS07

Signal Word

Danger

Hazard Statements

H315,H319,H334,H335

Precautionary Statements

P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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