Deoxyribonuclease I bovine

Description

• Application: DNAse I from Sigma was used to treat nuclear lysate to obtain single nucleosomes in a study.[1] The enzyme has also been for the preparation and harvest of mice mammary glands.[2]
• Biochem/physiol Actions: DNase I is an endonuclease that acts on phosphodiester bonds (adjacent to pyrimidines) to produce polynucleotides with terminal 5′-phosphates. The pH optimum is found to be between 7 and 8. Divalent cations such as Mn2+, Ca2+, Co2+, and Zn2+ are activators of the enzyme. A concentration of 5 mM Ca2+ stabilizes the enzyme against proteolytic digestion.[3] 2-Mercaptoethanol, chelators, sodium dodecyl sulfate (SDS)[4] and actin[5] are known to inhibit the enzyme activity.
• Features and Benefits: RNA purification by removing DNAPrepare DNA for nick translation1Footprinting assays to determine DNA-protein interactions2
• Unit Definition: One unit will produce a ΔA260 of 0.001 per min per mL reaction mixture using calf thymus DNA at pH 5.0 and 25°C
• Physical form: Supplied as a solution in 4 mg/ml glycine pH 5.0, 5 mM calcium acetate and 50% glycerol
• Preparation Note: Produced without using any animal cells or animal derived materials.