Endoglycosidase F1 from Elizabethkingia miricola

Description

• General description: Endoglycosidase F1 from Elizabethkingia miricola is a glycan specific enzyme.[1]
• Application: Endoglycosidase F1 from Elizabethkingia miricola has been used to remove binding of human hemochromatosis protein (HFE) to cation independent mannose-6-phosphate receptor (CI-MPR)[2] and to glycosylate fucosylated N-glycopeptides.[3]
• Biochem/physiol Actions: Endoglycosidase F1 from Elizabethkingia miricola plays an important role in cleavage of glycan structures from the protein by cleaving between the two N-acetylglucosamine residues of the chitobiose core. It mediates high mannose and hybrid oligosaccharides cleavage.[4]
• Packaging: Supplied with 5× Reaction Buffer, 250 mM NaH2PO4, pH 5.5.
• Unit Definition: One unit will release N-linked oligosaccharides from 1 μmole of denatured Ribonuclease B in 1 minute at 37 °C, pH 5.5.
• Physical form: Aseptically filled solution in 20 mM Tris-HCl pH 7.5