Description

• General description: β-Galactosidase is a member of the glycoside hydrolase clan A (GH-A) family. It is widely present in microorganisms, which includes both prokaryotes and eukaryotes.[1]
• Application: β-Galactosidase from Aspergillus oryzae has been used:in in vitro nuclear magnet resonance (NMR)[2]as a standard in the β-galactosidase assay to determine the concentration of cell-associated β-galactosidase[3]to modify sugar residues on the surface of the mutant slime cells to study the adhesive properties[4]for enzymatic hydrolysis of lactose[5]for reversed-phase (RP) adsorption[6]in the hydrolysis of whey lactose[7]
• Biochem/physiol Actions: β-Galactosidase acts on the terminal β-D-galactosyl moieties of disaccharides, glycoconjugates, and polysaccharides. It is useful in mediating transglycosylation reactions such as the synthesis of galactooligosaccharides (GOS). β-Galactosidases might be used for glycobiological and biotechnological applications.[1]
• Unit Definition: One unit will hydrolyze 1.0 μmole of lactose per minute at pH at 4.5 at 30 °C.