Protease and Phosphatase Inhibitor Cocktail

Description

• General description: Mass spectrometry (MS) compatible protease inhibitor cocktail and phosphatase inhibitor cocktail, with broad specificity for the inhibition of:Serine, cysteine and aspartic proteases, and metalloproteasesTyrosine, serine/threonine, acid and alkaline phosphatasesAll inhibitors and fillers were chosen so as not to interfere with LC-MS analyses. Any inhibitors capable of covalent, irreversible protein modification were avoided. This cocktail is EDTA-free. All inhibitors were selected to allow facile downstream sample processing, such as immobilized metal affinity chromatography (IMAC) for His-tagged protein purification and, uniquely, phosphopeptide enrichment.
• Specificity: Protease inhibitors: serine, cysteine and aspartic proteases, and metalloproteasesPhosphatase inhibitors: tyrosine, serine/threonine, acid and alkaline phosphatases
• Application: MS-SAFE Protease and Phosphatase Inhibitor has been used to measure ribosomal protein S6 kinase (S6K) activity.[1]
• Features and Benefits: Comprehensive protease and phosphatase inhibitor cocktail for mass spectrometry analysis Compatible with downstream sample processing such as His-tagged protein purification and phosphopeptide enrichment Allows accurate measurement of protein activity and identification of phosphorylation sites.
• Components: Protease inhibitors:Bestatin hydrochlorideLeupeptinPhosphoramidon disodium saltPepstatin AElastatinalAprotininNafamostat mesylateAntipainPhosphatase inhibitorsOkadaic acidSodium fluorideSodium orthovanadateBromotetramisole oxalateFillersβ-lactoseDL-leucine
• Quantity: One vial makes 20 mL of 1× inhibitor cocktail working solution, using either water or extraction/lysis buffer. Alternatively, a 10× concentrated solution may be prepared by adding 2 mL of water or extraction/lysis buffer. This 10× solution may then be diluted 10-fold into extraction/lysis buffer as needed for a 1× working solution.
• Physical form: Lyophilized powder that is water-soluble