Prolyl oligopeptidase

Description

• General description: Prolyl oligopeptidase (PO) is mapped to human chromosome 6q22.[1] It comprises of a N-terminal β-propeller domain and C-terminal α/β hydrolase catalytic domain.[2] PO is highly expressed in the neuronal cytoplasm and its expression increases with age.[3]
• Application: Prolyl oligopeptidase has been used in a study to assess the mechanism of lithium ion action. [5] It has also been used in a study to investigate its distribution in human tissue and body fluids. [6]
• Biochem/physiol Actions: Prolyl oligopeptidase (PO) regulates physiological processes and is crucial for the generation of active hormone and peptide fragments from their precursors. It shows elevated levels in psychiatric disorders and Alzheimer′s patients.[8] Altered levels of PO is observed in patients with bipolar disorder.[1] High expression of PO may promote metastasis of malignant ovarian and colorectal tumors.[9]
• Unit Definition: One unit will hydrolyze 1.0 picomole of Ala-Pro-aminomethylcoumarin per minute at pH 7.4 at 25 °C.
• Physical form: Supplied as a solution in 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 10% glycerol, 3 mM DTT and variable amounts of imidazole.
• Preparation Note: N-terminal GST-tagged 81.6 kDa full-length protein