Description

• General description: Thermolysin is a protease that has specificity different from other proteases available for sequence investigations. [1]
• Application: Non-specific Protease Activity Assay Video
• Quality: Contains many extraneous enzymes.
• Unit Definition: One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).
• Physical form: lyophilized powder containing calcium and sodium acetate buffer salts
• Preparation Note: The pH optimum is 8.0 and the optimal temperature for activity is 70 °C. Considerably stable from pH 5 to 9.5. Thermolysin has a low cleavage specificity, therefore, it produces a number of short fragments that are suitable for sequencing. Preferential cleavage: X-cleavage-Y-Z where X=any amino acid; Y=Leu, Phe, Ile, Val, Met, Ala and Z is any amino acid other than Pro. Cleavage N-terminal to Leu is preferred over cleavage of N-terminal to Phe which is preferred over the others.