P3804

o-Phenylenediamine dihydrochloride

chromogenic, tablet

Manufacturer: Sigma Aldrich

CAS Number: 615-28-1

Synonym(S): 1,2-Phenylenediamine tablet, OPD, OPD Tablet

Select a Size

Pack Size SKU Availability Price
50 TABS P3804-50-TABS In Stock ₹ 17,404.80
100 TABS P3804-100-TABS In Stock ₹ 30,003.30

P3804 - 50 TABS

₹ 17,404.80

In Stock

Quantity

1

Base Price: ₹ 17,404.80

GST (18%): ₹ 3,132.864

Total Price: ₹ 20,537.664

product name

o-Phenylenediamine dihydrochloride, tablet, 5 mg substrate per tablet

Quality Level

200

form

tablet

mp

258 °C (dec.) (lit.)

solubility

water: 1 tablet/10 mL, clear, colorless

storage temp.

2-8°C

SMILES string

Cl[H].Cl[H].Nc1ccccc1N

InChI

1S/C6H8N2.2ClH/c7-5-3-1-2-4-6(5)8;;/h1-4H,7-8H2;2*1H

InChI key

RIIWUGSYXOBDMC-UHFFFAOYSA-N

Other Options

Image Product Name Manufacturer Price Range
50-180-4583
Sigma Aldrich Fine Chemicals Biosciences o-Phenylenediamine dihydrochloride tablet, 5 mg substrate per tablet | 615-28-1 | MFCD00012966 | 50TAB
Sigma Aldrich Fine Chemicals Biosciences ₹ 23,792.52
50-180-4591
Sigma Aldrich Fine Chemicals Biosciences o-Phenylenediamine dihydrochloride tablet, 20 mg substrate per tablet | 615-28-1 | MFCD00012966 | 100TAB
Sigma Aldrich Fine Chemicals Biosciences ₹ 48,769.20
50-180-4599
Sigma Aldrich Fine Chemicals Biosciences o-Phenylenediamine dihydrochloride tablet, 3 mg substrate per tablet | 615-28-1 | MFCD00012966 | 50TAB
Sigma Aldrich Fine Chemicals Biosciences ₹ 22,848.80
50-180-4586
Sigma Aldrich Fine Chemicals Biosciences o-Phenylenediamine dihydrochloride tablet, 1 mg substrate per tablet | 615-28-1 | MFCD00012966 | 100TAB
Sigma Aldrich Fine Chemicals Biosciences ₹ 37,941.58
50-180-4594
Sigma Aldrich Fine Chemicals Biosciences o-Phenylenediamine dihydrochloride tablet, 10 mg substrate per tablet | 615-28-1 | MFCD00012966 | 50TAB
Sigma Aldrich Fine Chemicals Biosciences ₹ 27,146.48
50-180-4578
Sigma Aldrich Fine Chemicals Biosciences o-Phenylenediamine dihydrochloride peroxidase substrate | 615-28-1 | MFCD00012966 | 100G
Sigma Aldrich Fine Chemicals Biosciences ₹ 71,985.05
50-180-4579
Sigma Aldrich Fine Chemicals Biosciences o-Phenylenediamine dihydrochloride peroxidase substrate | 615-28-1 | MFCD00012966 | 10G
Sigma Aldrich Fine Chemicals Biosciences ₹ 12,577.32
50-180-4584
Sigma Aldrich Fine Chemicals Biosciences o-Phenylenediamine dihydrochloride tablet, 15 mg substrate per tablet | 615-28-1 | MFCD00012966 | 100TAB
Sigma Aldrich Fine Chemicals Biosciences ₹ 46,818.43
50-180-4589
Sigma Aldrich Fine Chemicals Biosciences o-Phenylenediamine dihydrochloride tablet, 2 mg substrate per tablet | 615-28-1 | MFCD00012966 | 50TAB
Sigma Aldrich Fine Chemicals Biosciences ₹ 20,333.33
50-180-4592
Sigma Aldrich Fine Chemicals Biosciences o-Phenylenediamine dihydrochloride tablet, 20 mg substrate per tablet | 615-28-1 | MFCD00012966 | 50TAB
Sigma Aldrich Fine Chemicals Biosciences ₹ 29,137.46

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Description

  • General description: o-Phenylenediamine dihydrochloride (OPD) is a chromogenic substrate that can be used in enzyme-linked immunosorbent assay (ELISA) procedures as it utilizes horseradish peroxidase conjugates. The substrate produces a soluble end product that is orange-brown and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3N HCl or 3M H2SO4 and read at 492 nm.
  • Application: o-Phenylenediamine dihydrochloride has been used:as a chromogenic substrate in indirect enzyme-linked immunosorbent assay (ELISA) to detect immunoglobin G (IgG) antibodies against certain recombinant antigens/proteinsas a streptavidin-horseradish peroxidase (HRP) substrate sandwich ELISA to analyze human interleukin (IL)-17A, IL-23, and IP-10as a substrate in ELISA for determination of IgG-class antibodies to Mycobacterium bovis in serum
  • Packaging: 16 mg total tablet weight
  • Reconstitution: Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.

SAFETY INFORMATION

Pictograms

GHS08,GHS07,GHS09

Signal Word

Warning

Hazard Statements

H302 + H332,H317,H319,H341,H351,H410

Precautionary Statements

P273 - P280 - P301 + P312 - P302 + P352 - P304 + P340 + P312 - P308 + P313

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Sens. 1

WGK

WGK 3

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o-Phenylenediamine dihydrochloride, tablet, 5 mg substrate per tablet

Quality Level:
200

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258 °C (dec.) (lit.)

solubility:
water: 1 tablet/10 mL, clear, colorless

storage temp.:
2-8°C

SMILES string:
Cl[H].Cl[H].Nc1ccccc1N

InChI:
1S/C6H8N2.2ClH/c7-5-3-1-2-4-6(5)8;;/h1-4H,7-8H2;2*1H

InChI key:
RIIWUGSYXOBDMC-UHFFFAOYSA-N

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