P5994

Phosphatase substrate

40 mg tablets

Manufacturer: Sigma Aldrich

CAS Number: 333338-18-4

Synonym(S): 4-Nitrophenyl phosphate disodium salt hexahydrate, pNPP disodium salt hexahydrate

Select a Size

Pack Size SKU Availability Price
25 TABS P5994-25-TABS In Stock ₹ 17,406.60

P5994 - 25 TABS

₹ 17,406.60

In Stock

Quantity

1

Base Price: ₹ 17,406.60

GST (18%): ₹ 3,133.188

Total Price: ₹ 20,539.788

Quality Level

200

form

tablet

concentration

36-44 mg/tablet

impurities

p-nitrophenol, essentially free

solubility

H2O: soluble, clear, colorless to yellow (1 tablet in 8 mL of water.)

storage temp.

−20°C

SMILES string

O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1

InChI

1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2

InChI key

KAKKHKRHCKCAGH-UHFFFAOYSA-L

Other Options

Image Product Name Manufacturer Price Range
50-179-0002
Sigma Aldrich Fine Chemicals Biosciences 4-Nitrophenyl phosphate disodium salt hexahydrate | 333338-18-4 | MFCD00066288 | 100 tablets
Sigma Aldrich Fine Chemicals Biosciences ₹ 41,191.15
50-179-0003
Sigma Aldrich Fine Chemicals Biosciences 4-Nitrophenyl phosphate disodium salt hexahydrate tablet | 333338-18-4 | MFCD00066288 | 50TAB
Sigma Aldrich Fine Chemicals Biosciences ₹ 24,106.53
50-179-0013
Sigma Aldrich Fine Chemicals Biosciences Phosphatase Substrate | 333338-18-4 | MFCD00066288 | 200Tab
Sigma Aldrich Fine Chemicals Biosciences ₹ 19,285.22
50-179-0014
Sigma Aldrich Fine Chemicals Biosciences Phosphatase substrate | 333338-18-4 | MFCD00066288 | 50TAB
Sigma Aldrich Fine Chemicals Biosciences ₹ 6,579.56
50-178-9994
Sigma Aldrich Fine Chemicals Biosciences 4-Nitrophenyl phosphate disodium salt hexahydrate suitable for enzyme immunoassay, >=99.0% (enzymatic) | 333338-18-4 | MFCD00066288 | 25G
Sigma Aldrich Fine Chemicals Biosciences ₹ 55,956.24
50-178-9995
Sigma Aldrich Fine Chemicals Biosciences 4-Nitrophenyl phosphate disodium salt hexahydrate | 333338-18-4 | MFCD00066288 | 5g
Sigma Aldrich Fine Chemicals Biosciences ₹ 14,359.53
50-179-0012
Sigma Aldrich Fine Chemicals Biosciences Phosphatase substrate 5 mg tablets | 333338-18-4 | MFCD00066288 | 100TAB
Sigma Aldrich Fine Chemicals Biosciences ₹ 10,900.34
NC1004850
Sigma Aldrich Fine Chemicals Biosciences Phosphatase substrate powder | 333338-18-4 | MFCD00066288 | 5G
Sigma Aldrich Fine Chemicals Biosciences ₹ 43,122.24
S0942
Phosphatase substrate
Sigma Aldrich ₹ 5,921.28 - ₹ 18,759.73
71768
4-Nitrophenyl phosphate disodium salt hexahydrate
Sigma Aldrich ₹ 8,411.03 - ₹ 31,251.78

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Description

  • General description: Human phosphatase-substrate networks are mapped based on the literature and protein-protein interaction (PPI) database. Based on gene expression data, the possible network of phosphatase-substrate-kinase is predicted.[1]
  • Application: Phosphatase is a chromogenic substrate for use with alkaline and acid phosphatase ELISA procedures. It is been used for the colorimetric detection of CTB fusion proteins[2]. Produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 N NaOH and read at 405 nm.
  • Quantity: Contains 40 mg substrate per tablet
  • Preparation Note: Dissolve tablets to the desired concentration in either of the following buffers: 0.1 M glycine, pH 10.4, with 1 mM MgCl2 and 1 mM ZnCl2 or 1 M diethanolamine, pH 9.8, with 0.5 mM MgCl2. Typically, a pNPP concentration of 1 mg/mL is used.
  • Other Notes: Substrate for the determination of acid and alkaline phosphatases.

SAFETY INFORMATION

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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tablet

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impurities:
p-nitrophenol, essentially free

solubility:
H2O: soluble, clear, colorless to yellow (1 tablet in 8 mL of water.)

storage temp.:
−20°C

SMILES string:
O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1

InChI:
1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2

InChI key:
KAKKHKRHCKCAGH-UHFFFAOYSA-L

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Nc1ccc(N)cc1

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1S/C6H8N2/c7-5-1-2-6(8)4-3-5/h1-4H,7-8H2

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__

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__